Rat TRKB (Tyrosine Kinase B) ELISA Kit | MyBioSource MBS2512413 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rat TRKB (Tyrosine Kinase B) ELISA Kit

catalog :

MBS2512413

quantity :

48-Strip-Wells

price :

440 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2512413

products type :

ELISA Kit

products full name :

Rat TRKB (Tyrosine Kinase B) ELISA Kit

products short name :

TRKB

other names :

Tropomyosin 3; Tropomyosin alpha-3 chain; tropomyosin alpha-3 chain; alpha-tropomyosin, slow skeletal; cytoskeletal tropomyosin TM30; epididymis luminal protein 189; epididymis secretory sperm binding protein Li 82p; heat-stable cytoskeletal protein 30 kDa; tropomyosin gamma; tropomyosin-5; tropomyosin 3; Gamma-tropomyosin; Tropomyosin-3; Tropomyosin-5; hTM5

products gene name :

TRKB

other gene names :

TPM3; TPM3; TM3; TM5; TRK; CFTD; NEM1; TM-5; TM30; CAPM1; TM30nm; TPMsk3; hscp30; HEL-189; HEL-S-82p; OK/SW-cl.5; hTM5

uniprot entry name :

TPM3_HUMAN

reactivity :

Rat

sequence length :

248

specificity :

This kit recognizes natural and recombinant Rat TRKB. No significant cross-reactivity or interference between Rat TRKB and analogues was observed.

storage stability :

Store at 4 degree C.

other info1 :

Samples: Serum, plasma and other biological fluids. Assay Type: Sandwich. Detection Range: 93.75-6000pg/mL. Sensitivity: The minimum detectable dose of Rat TRKB is 56.25pg/mL (The sensitivity of this assay, or lowest detectable limit (LDL) was defined as the lowest protein concentration that could be differentiated from zero).

other info2 :

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Rat TRKB concentrations in serum, plasma and other biological fluids.

products description :

Principle of the assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to TRKB. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for TRKB and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain TRKB, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of TRKB. You can calculate the concentration of TRKB in the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

47938162

ncbi acc num :

AAH72428.1

uniprot acc num :

P06753

ncbi mol weight :

18,603 Da

ncbi pathways :

Adrenergic Signaling In Cardiomyocytes Pathway (908257); Adrenergic Signaling In Cardiomyocytes Pathway (909696); Cardiac Muscle Contraction Pathway (93344); Cardiac Muscle Contraction Pathway (93992); Dilated Cardiomyopathy Pathway (121494); Dilated Cardiomyopathy Pathway (121285); Hypertrophic Cardiomyopathy (HCM) Pathway (114229); Hypertrophic Cardiomyopathy (HCM) Pathway (106591); Muscle Contraction Pathway (106261); Pathways In Cancer (83105)

ncbi summary :

This gene encodes a member of the tropomyosin family of actin-binding proteins. Tropomyosins are dimers of coiled-coil proteins that provide stability to actin filaments and regulate access of other actin-binding proteins. Mutations in this gene result in autosomal dominant nemaline myopathy and other muscle disorders. This locus is involved in translocations with other loci, including anaplastic lymphoma receptor tyrosine kinase (ALK) and neurotrophic tyrosine kinase receptor type 1 (NTRK1), which result in the formation of fusion proteins that act as oncogenes. There are numerous pseudogenes for this gene on different chromosomes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2013]

uniprot summary :

TPM3: a cytoskeletal protein that binds to actin filaments in muscle and nonmuscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments. Defects in TPM3 are a cause of nemaline myopathy type 1 (NEM1). Three alternatively spliced isoforms have been described. Protein type: Motility/polarity/chemotaxis; Motor; Oncoprotein; Contractile; Actin-binding; Cytoskeletal. Chromosomal Location of Human Ortholog: 1q21.2. Cellular Component: cortical cytoskeleton; filamentous actin; growth cone; cytoskeleton; stress fiber; podosome; muscle thin filament tropomyosin; cytosol; cleavage furrow. Molecular Function: actin binding. Biological Process: muscle contraction; cell motility; muscle filament sliding. Disease: Nemaline Myopathy 1; Myopathy, Congenital, With Fiber-type Disproportion

size1 :

48-Strip-Wells

price1 :

440 USD

size2 :

96-Strip-Wells

price2 :

530

more info or order :

MyBioSource product webpage