Porcine TRAIL/TNFSF10 (Tumor Necrosis Factor Related Apoptosis Inducing Ligand) ELISA Kit | MyBioSource MBS2512220 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Porcine TRAIL/TNFSF10 (Tumor Necrosis Factor Related Apoptosis Inducing Ligand) ELISA Kit

catalog :

MBS2512220

quantity :

24-Strip-Wells

price :

235 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2512220

products type :

ELISA Kit

products full name :

Porcine TRAIL/TNFSF10 (Tumor Necrosis Factor Related Apoptosis Inducing Ligand) ELISA Kit

products short name :

[TRAIL/TNFSF10]

other names :

[tumor necrosis factor ligand superfamily member 10 isoform 1; Tumor necrosis factor ligand superfamily member 10; tumor necrosis factor ligand superfamily member 10; Apo-2 ligand; TNF-related apoptosis inducing ligand TRAIL; chemokine tumor necrosis factor ligand superfamily member 10; tumor necrosis factor (ligand) family, member 10; tumor necrosis factor apoptosis-inducing ligand splice variant delta; tumor necrosis factor (ligand) superfamily, member 10; Apo-2 ligand; Apo-2L; TNF-related apoptosis-inducing ligand; Protein TRAIL; CD_antigen: CD253]

products gene name :

[TRAIL/TNFSF10]

other gene names :

[TNFSF10; TNFSF10; TL2; APO2L; CD253; TRAIL; Apo-2L; APO2L; TRAIL; Apo-2L; Protein TRAIL]

uniprot entry name :

TNF10_HUMAN

reactivity :

Porcine

sequence length :

281

specificity :

This kit recognizes Porcine Tn-I in samples. No significant cross-reactivity or interference between PorcineTn-I and analogues was observed.

storage stability :

Store at 4 degree C.

other info1 :

Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 31.25-2000pg/mL. Sensitivity: 18.75pg/mL.

products description :

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Porcine Tn-I concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Porcine Tn-I. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Porcine Tn-I and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Porcine Tn-I, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-subst rate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Porcine Tn-I. You can calculate the concentration of Porcine Tn-I in the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

4507593

ncbi acc num :

NP_003801.1

ncbi gb acc num :

NM_003810.3

uniprot acc num :

P50591

ncbi mol weight :

11,746 Da

ncbi pathways :

Apoptosis Pathway (198797); Apoptosis Pathway (83060); Apoptosis Pathway (470); Apoptosis Pathway (105648); Apoptosis Modulation And Signaling Pathway (198822); Caspase-8 Activation By Cleavage Pathway (105654); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Death Receptor Signalling Pathway (105650); Dimerization Of Procaspase-8 Pathway (105655)

ncbi summary :

The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This protein preferentially induces apoptosis in transformed and tumor cells, but does not appear to kill normal cells although it is expressed at a significant level in most normal tissues. This protein binds to several members of TNF receptor superfamily including TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and possibly also to TNFRSF11B/OPG. The activity of this protein may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and TNFRSF11B/OPG that cannot induce apoptosis. The binding of this protein to its receptors has been shown to trigger the activation of MAPK8/JNK, caspase 8, and caspase 3. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2010]

uniprot summary :

TRAIL: Cytokine that binds to TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and possibly also to TNFRSF11B/OPG. Induces apoptosis. Its activity may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and TNFRSF11B/OPG that cannot induce apoptosis. Homotrimer. Widespread; most predominant in spleen, lung and prostate. Belongs to the tumor necrosis factor family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral; Cytokine. Chromosomal Location of Human Ortholog: 3q26. Cellular Component: extracellular space; integral to plasma membrane; extracellular region. Molecular Function: protein binding; metal ion binding; cytokine activity; tumor necrosis factor receptor binding; receptor binding. Biological Process: caspase activation; positive regulation of I-kappaB kinase/NF-kappaB cascade; cell-cell signaling; apoptosis; positive regulation of apoptosis; male gonad development; positive regulation of caspase activity; immune response; signal transduction; response to insulin stimulus

size1 :

24-Strip-Wells

price1 :

235 USD

size2 :

48-Strip-Wells

price2 :

450

size3 :

96-Strip-Wells

price3 :

535

size4 :

5x96-Strip-Wells

price4 :

2255

size5 :

10x96-Strip-Wells

price5 :

3975

more info or order :

MyBioSource product webpage