ELISA/assay

LXA4 (Lipoxin A4) ELISA Kit

MBS2512197

96-Strip-Wells

445 USD

ELISA Kit

LXA4 (Lipoxin A4) ELISA Kit

LXA4

lipoxin A4 receptor; N-formyl peptide receptor 2; N-formyl peptide receptor 2; FMLP-R-I; FMLP-related receptor I; LXA4 receptor; RFP; formyl peptide receptor-like 1; lipoxin A4 receptor (formyl peptide receptor related); formyl peptide receptor 2; FMLP-related receptor I; FMLP-R-I; Formyl peptide receptor-like 1; HM63; Lipoxin A4 receptor; LXA4 receptor; RFP

LXA4

FPR2; FPR2; ALXR; HM63; FMLPX; FPR2A; FPRH1; FPRH2; FPRL1; LXA4R; FMLP-R-II; FPRH1; FPRL1; LXA4R; FMLP-R-I; LXA4 receptor

FPR2_HUMAN

All

351

This kit recognizes natural and recombinant LXA4. No significant cross-reactivity or interference between LXA4 and analogues was observed.

Store at 4 degree C.

Samples: Serum, plasma and other biological fluids. Assay Type: Competitive. Detection Range: 0.781-50ng/mL. Sensitivity: 0.469ng/mL

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of LXA4 concentrations in serum, plasma and other biological fluids. Principle of the Assay This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with LXA4. During the reaction, LXA4 in the sample or standard competes with a fixed amount of LXA4 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to LXA4. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of LXA4 in the samples is then determined by comparing the OD of the samples to the standard curve.

3047269

AAC13684.1

P25090

38,964 Da

Class A/1 (Rhodopsin-like Receptors) Pathway (106357); Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway (1127664); Disease Pathway (530764); Formyl Peptide Receptors Bind Formyl Peptides And Many Other Ligands Pathway (161021); G Alpha (i) Signalling Events Pathway (119550); G Alpha (q) Signalling Events Pathway (106043); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020); GPCRs, Class A Rhodopsin-like Pathway (198886); Gastrin-CREB Signalling Pathway Via PKC And MAPK (645295)

FPR2: Low affinity receptor for N-formyl-methionyl peptides, which are powerful neutrophils chemotactic factors. Binding of FMLP to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system. The activation of LXA4R could result in an anti-inflammatory outcome counteracting the actions of proinflammatory signals such as LTB4 (leukotriene B4). Belongs to the G-protein coupled receptor 1 family. Protein type: Receptor, GPCR; Membrane protein, multi-pass; Membrane protein, integral; Motility/polarity/chemotaxis; GPCR, family 1. Chromosomal Location of Human Ortholog: 19q13.3-q13.4. Cellular Component: integral to membrane; plasma membrane. Molecular Function: G-protein coupled receptor activity; N-formyl peptide receptor activity. Biological Process: G-protein coupled receptor protein signaling pathway; cell motility; chemotaxis; cell adhesion; inflammatory response

96-Strip-Wells

445 USD