catalog number :
MBS2512159
products type :
ELISA Kit
products full name :
Human ACE (Angiotensin I Converting Enzyme) ELISA Kit
products short name :
[ACE]
other names :
[Ace; Accessory cholera enterotoxin]
products gene name :
[ACE]
other gene names :
[ace; Ace]
uniprot entry name :
ACE_VIBCH
specificity :
This kit recognizes Human I CTP in samples. No significant cross-reactivity or interference between Human ICTP and analogues was observed.
storage stability :
Store at 4 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 7.81-500ng/mL. Sensitivity: 4.69ng/mL.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human ICTP were tested 20 times on one plate, respectively. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human ICTP were tested on 3 different plates, 20 replicates in each plate.
products description :
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human ICTP concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ICTP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ICTP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ICTP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human ICTP. You can calculate the concentration of Human ICTP in the samples by comparing the OD of the samples to the standard curve.
ncbi acc num :
CAA80292.1
ncbi mol weight :
11,347 Da
uniprot summary :
Increases short-circuit current in rabbit ileal tissue mounted in Ussing chambers, by increasing the potential difference. Cultures of V.cholerae containing the cloned ace gene cause fluid secretion in ligated rabbit ileal loops.
size5 :
10x96-Strip-Wells