Rat M-CSF (Macrophage Colony-Stimulating Factor) ELISA Kit | MyBioSource MBS2512155 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rat M-CSF (Macrophage Colony-Stimulating Factor) ELISA Kit

catalog :

MBS2512155

quantity :

24-Strip-Wells

price :

215 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS2512155

products type :

ELISA Kit

products full name :

Rat M-CSF (Macrophage Colony-Stimulating Factor) ELISA Kit

products short name :

[M-CSF]

other names :

[macrophage colony-stimulating factor 1 receptor; Macrophage colony-stimulating factor 1 receptor; macrophage colony-stimulating factor 1 receptor; CSF-1 receptor; CSF-1-R; c-fms; proto-oncogene c-Fms; proto-oncogene fms; CSF-1 receptor (EC:2.7.10.1); CSF-1-R; CSF-1R; M-CSF-R; Proto-oncogene c-Fms; CD_antigen: CD115]

products gene name :

[M-CSF]

other gene names :

[CSF1R; CSF1R; CSF1; M-CSF; CSF-1R; M-CSF-R; FMS; CSF-1-R; CSF-1R; M-CSF-R]

uniprot entry name :

CSF1R_FELCA

reactivity :

Rat

sequence length :

980

specificity :

This kit recognizes Rat IL-11 in samples. No significant cross-reactivity or interference between Rat IL-11 and analogues was observed.

storage stability :

Store at 4 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 15.63-1000pg/mL. Sensitivity: 9.38pg/mL.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, midrange and high level Rat IL-11 were tested 20times on one plate, respectively. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, midrange and high level Rat IL-11 were testedon 3 different plates, 20 replicates in each plate.

products description :

Intended Uses: This ELISA kit can be applied to the in vitro quantitative determination of Rat IL-11 concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat IL-11. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Rat IL-11 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat IL-11, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/-2 nm. The OD value is proportional to the concentration of Rat IL-11. You can calculate the concentration of Rat IL-11 in the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

57163771

ncbi acc num :

NP_001009231.1

ncbi gb acc num :

NM_001009231.1

uniprot acc num :

P13369

ncbi mol weight :

108,507 Da

ncbi pathways :

Cytokine-cytokine Receptor Interaction Pathway (741721); Cytokine-cytokine Receptor Interaction Pathway (460); Endocytosis Pathway (741733); Endocytosis Pathway (102181); Hematopoietic Cell Lineage Pathway (741764); Hematopoietic Cell Lineage Pathway (489); Osteoclast Differentiation Pathway (741749); Osteoclast Differentiation Pathway (193096); PI3K-Akt Signaling Pathway (741738); PI3K-Akt Signaling Pathway (692979)

uniprot summary :

Tyrosine-protein kinase that acts as cell-surface receptor for CSF1 and IL34 and plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines in response to IL34 and CSF1, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone and tooth development. Required for normal male and female fertility, and for normal development of milk ducts and acinar structures in the mammary gland during pregnancy. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration, and promotes cancer cell invasion. Activates several signaling pathways in response to ligand binding. Phosphorylates PIK3R1, PLCG2, GRB2, SLA2 and CBL. Activation of PLCG2 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate, that then lead to the activation of protein kinase C family members, especially PRKCD. Phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, leads to activation of the AKT1 signaling pathway. Activated CSF1R also mediates activation of the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1, and of the SRC family kinases SRC, FYN and YES1. Activated CSF1R transmits signals both via proteins that directly interact with phosphorylated tyrosine residues in its intracellular domain, or via adapter proteins, such as GRB2. Promotes activation of STAT family members STAT3, STAT5A and/or STAT5B. Promotes tyrosine phosphorylation of SHC1 and INPP5D/SHIP-1. Receptor signaling is down-regulated by protein phosphatases, such as INPP5D/SHIP-1, that dephosphorylate the receptor and its downstream effectors, and by rapid internalization of the activated receptor ().

size1 :

24-Strip-Wells

price1 :

215 USD

size2 :

48-Strip-Wells

price2 :

410

size3 :

96-Strip-Wells

price3 :

490

size4 :

5x96-Strip-Wells

price4 :

2040

size5 :

10x96-Strip-Wells

price5 :

3590

more info or order :

MyBioSource product webpage