ELISA/assay

Rat HNF1a (Hepatocyte Nuclear Factor 1 Alpha) ELISA Kit

MBS2512083

24-Strip-Wells

215 USD

ELISA Kit

Rat HNF1a (Hepatocyte Nuclear Factor 1 Alpha) ELISA Kit

[HNF1a]

[hepatocyte nuclear factor 1-alpha; Hepatocyte nuclear factor 1-alpha; hepatocyte nuclear factor 1-alpha; HNF-1-alpha; albumin proximal factor; hepatic nuclear factor 1; interferon production regulator factor; liver-specific transcription factor LF-B1; transcription factor 1, hepatic; HNF1 homeobox A; Liver-specific transcription factor LF-B1; LFB1; Transcription factor 1; TCF-1]

[HNF1a]

[HNF1A; HNF1A; HNF1; LFB1; TCF1; MODY3; TCF-1; HNF-1A; IDDM20; TCF1; HNF-1-alpha; HNF-1A; LFB1; TCF-1]

HNF1A_HUMAN

Rat

631

This kit recognizes natural and recombinantRatHNF1a. No significant cross-reactivity or interference between RatHNF1a and analogues was observed.

Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 0.156-10ng/mL. Sensitivity: Min: 0.094ng/mL; Max: 10ng/mL

Intended Uses: This ELISA kit applies to the invitro quantitative determination of RatHNF1aconcentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to HNF1a. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for HNF1aand Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain HNF1a, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of HNF1a.You can calculate the concentration of HNF1ain the samples by comparing the OD of the samples to the standard curve.

256542297

NP_000536.5

NM_000545.5

P20823

12,491 Da

Adipogenesis Pathway (198832); Developmental Biology Pathway (477129); FOXA2 And FOXA3 Transcription Factor Networks Pathway (137911); IL-6 Signaling Pathway (198864); Maturity Onset Diabetes Of The Young Pathway (83096); Maturity Onset Diabetes Of The Young Pathway (508); Presenilin Action In Notch And Wnt Signaling Pathway (137914); Regulation Of Beta-cell Development Pathway (106328); Regulation Of Gene Expression In Beta Cells Pathway (106332); Signaling Pathways Regulating Pluripotency Of Stem Cells (1026136)

The protein encoded by this gene is a transcription factor required for the expression of several liver-specific genes. The encoded protein functions as a homodimer and binds to the inverted palindrome 5'-GTTAATNATTAAC-3'. Defects in this gene are a cause of maturity onset diabetes of the young type 3 (MODY3) and also can result in the appearance of hepatic adenomas. [provided by RefSeq, Mar 2009]

HNF1A: Transcriptional activator that regulates the tissue specific expression of multiple genes, especially in pancreatic islet cells and in liver. Required for the expression of several liver specific genes. Binds to the inverted palindrome 5 - GTTAATNATTAAC-3 . Defects in HNF1A are a cause of hepatic adenomas familial (HEPAF). Hepatic adenomas are rare benign liver tumors of presumable epithelial origin that develop in an otherwise normal liver. Hepatic adenomas may be single or multiple. They consist of sheets of well-differentiated hepatocytes that contain fat and glycogen and can produce bile. Bile ducts or portal areas are absent. Kupffer cells, if present, are reduced in number and are non-functional. Conditions associated with adenomas are insulin-dependent diabetes mellitus and glycogen storage diseases (types 1 and 3). Bi-allelic inactivation of HNF1A, whether sporadic or associated with MODY3, may be an early step in the developmant of some hepatocellular carcinomas. Defects in HNF1A are the cause of maturity-onset diabetes of the young type 3 (MODY3); also symbolized MODY-3. MODY is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease. Defects in HNF1A are the cause of susceptibility to diabetes mellitus insulin-dependent type 20 (IDDM20). IDDM20 is a multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These features can result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels. Belongs to the HNF1 homeobox family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: DNA-binding; Transcription factor. Chromosomal Location of Human Ortholog: 12q24.2. Cellular Component: nucleoplasm; protein complex; cytoplasm; nucleus. Molecular Function: protein dimerization activity; protein binding; protein homodimerization activity; DNA binding; protein heterodimerization activity; transcription factor activity. Biological Process: regulation of transcription from RNA polymerase II promoter; transcription, DNA-dependent; insulin secretion; positive regulation of transcription, DNA-dependent; glucose import; positive regulation of transcription from RNA polymerase II promoter; glucose homeostasis. Disease: Diabetes Mellitus, Insulin-dependent, 20; Diabetes Mellitus, Insulin-dependent; Hepatic Adenomas, Familial; Maturity-onset Diabetes Of The Young, Type 3; Renal Cell Carcinoma, Nonpapillary; Diabetes Mellitus, Noninsulin-dependent

24-Strip-Wells

215 USD

48-Strip-Wells

410

96-Strip-Wells

490

5x96-Strip-Wells

2040

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3590