catalog number :
MBS2512019
products type :
ELISA Kit
products full name :
Mouse LZM (Lysozyme) ELISA Kit
products short name :
[LZM]
other names :
[lysozyme C; Lysozyme C; lysozyme C; 1,4-beta-N-acetylmuramidase C; c-type lysozyme; lysozyme; 1,4-beta-N-acetylmuramidase C]
products gene name :
[LZM]
other gene names :
[LYZ; LYZ; LZM; LZM]
uniprot entry name :
LYSC_HUMAN
specificity :
This kit recognizes Mouse LIFR in samples. No Significant cross-reactivity or interference between Mouse LIFR and analogues was observed.
storage stability :
Store at 4 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 31.25-2000 pg/mL. Sensitivity: 18.75 pg/mL
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse LIFR were tested 20 times on one plate, respectively. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse LIFR were tested on 3 different plates, 20 replicates in each plate.
products description :
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Mouse LIFR concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse LIFR. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse LIFR and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse LIFR, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse LIFR. You can calculate the concentration of Mouse LIFR in the samples by comparing the OD of the samples to the standard curve.
ncbi acc num :
NP_000230.1
ncbi gb acc num :
NM_000239.2
ncbi mol weight :
16,537 Da
ncbi pathways :
Amyloids Pathway (366238); C-MYB Transcription Factor Network Pathway (138073); Disease Pathway (530764); Salivary Secretion Pathway (153376); Salivary Secretion Pathway (153352)
ncbi summary :
This gene encodes human lysozyme, whose natural substrate is the bacterial cell wall peptidoglycan (cleaving the beta[1-4]glycosidic linkages between N-acetylmuramic acid and N-acetylglucosamine). Lysozyme is one of the antimicrobial agents found in human milk, and is also present in spleen, lung, kidney, white blood cells, plasma, saliva, and tears. The protein has antibacterial activity against a number of bacterial species. Missense mutations in this gene have been identified in heritable renal amyloidosis. [provided by RefSeq, Oct 2014]
uniprot summary :
LYZ: Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte- macrophage system and enhance the activity of immunoagents. Defects in LYZ are a cause of amyloidosis type 8 (AMYL8); also known as systemic non-neuropathic amyloidosis or Ostertag-type amyloidosis. AMYL8 is a hereditary generalized amyloidosis due to deposition of apolipoprotein A1, fibrinogen and lysozyme amyloids. Viscera are particularly affected. There is no involvement of the nervous system. Clinical features include renal amyloidosis resulting in nephrotic syndrome, arterial hypertension, hepatosplenomegaly, cholestasis, petechial skin rash. Belongs to the glycosyl hydrolase 22 family. Protein type: Secreted, signal peptide; Secreted; Vesicle; Hydrolase; EC 3.2.1.17; Endoplasmic reticulum. Chromosomal Location of Human Ortholog: 12q15. Cellular Component: extracellular space; extracellular region. Molecular Function: identical protein binding; lysozyme activity. Biological Process: retinal homeostasis; metabolic process; cytolysis; defense response to bacterium; inflammatory response. Disease: Amyloidosis, Familial Visceral
size5 :
10x96-Strip-Wells