catalog number :
MBS2512006
products type :
ELISA Kit
products full name :
Porcine C4 (Complement Component 4) ELISA Kit
products short name :
[C4]
other names :
[C4; C4; C4]
products gene name :
[C4]
uniprot entry name :
I6YM32_CHRPC
specificity :
This kit recognizes natural and some recombinant Porcine C4. No Significant cross -reactivity or interference between Porcine C4 and analogues was observed.
storage stability :
Store at 4 degree C.
other info1 :
Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 7.813-500ng/mL. Sensitivity: Min: 4.688ng/mL; Max: 500ng/mL
products description :
Intended Uses: This ELISA kit can be applied to the in vitro quantitative determination of Porcine C4 concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to C4. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for C4and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain C4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of C4.You can calculate the concentration of C4in the samples by comparing the OD of the samples to the standard curve.
ncbi acc num :
AFN53623.1
ncbi mol weight :
187,154 Da
size5 :
10x96-Strip-Wells