Human IL-35 (Interleukin 35) ELISA Kit | MyBioSource MBS2511987 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human IL-35 (Interleukin 35) ELISA Kit

catalog :

MBS2511987

quantity :

48-Strip-Wells

price :

410 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2511987

products type :

ELISA Kit

products full name :

Human IL-35 (Interleukin 35) ELISA Kit

products short name :

IL-35

other names :

interleukin-27 subunit beta; Interleukin-27 subunit beta; interleukin-27 subunit beta; EBV-induced gene 3 protein; Epstein-Barr virus induced gene 3; IL-27 subunit beta; IL27 subunit; IL35 subunit; cytokine receptor; epstein-Barr virus-induced gene 3 protein; Epstein-Barr virus induced 3; Epstein-Barr virus-induced gene 3 protein; EBV-induced gene 3 protein

products gene name :

IL-35

other gene names :

EBI3; EBI3; IL27B; IL-27B; IL27B; IL-27 subunit beta; IL-27B; EBV-induced gene 3 protein

uniprot entry name :

IL27B_HUMAN

reactivity :

Human

sequence length :

229

specificity :

This kit recognizes natural and recombinant Human IL-35. No significant cross-reactivity or interference between Human IL-35 and analogues was observed.

storage stability :

Store at 4 degree C.

other info1 :

Samples: Serum, plasma and other biological fluids. Assay Type: Sandwich. Detection Range: 15.625-1000pg/mL. Sensitivity: The minimum detectable dose of Human IL-35 is 9.375pg/mL

products description :

Intended Uses This ELISA kit applies to the in vitro quantitative determination of Human IL-35 concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to IL-35. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for IL-35 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain IL-35, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of IL-35. You can calculate the concentration of IL-35 in the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

14577917

ncbi acc num :

NP_005746.2

ncbi gb acc num :

NM_005755.2

uniprot acc num :

Q14213

ncbi mol weight :

25,396 Da

ncbi pathways :

IL27-mediated Signaling Events Pathway (137929)

ncbi summary :

This gene was identified by its induced expression in B lymphocytes in response Epstein-Barr virus infection. It encodes a secreted glycoprotein belonging to the hematopoietin receptor family, and heterodimerizes with a 28 kDa protein to form interleukin 27 (IL-27). IL-27 regulates T cell and inflammatory responses, in part by activating the Jak/STAT pathway of CD4+ T cells. [provided by RefSeq, Sep 2008]

uniprot summary :

IL27-beta: Cytokine with pro- and anti-inflammatory properties, that can regulate T-helper cell development, suppress T-cell proliferation, stimulate cytotoxic T-cell activity, induce isotype switching in B-cells, and that has diverse effects on innate immune cells. Among its target cells are CD4 T-helper cells which can differentiate in type 1 effector cells (TH1), type 2 effector cells (TH2) and IL17 producing helper T-cells (TH17). It drives rapid clonal expansion of naive but not memory CD4 T-cells. It also strongly synergizes with IL-12 to trigger interferon- gamma/IFN-gamma production of naive CD4 T-cells, binds to the cytokine receptor WSX-1/TCCR. Another important role of IL27 is its antitumor activity as well as its antiangiogenic activity with activation of production of antiangiogenic chemokines. Belongs to the type I cytokine receptor family. Type 3 subfamily. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 19p13.3. Cellular Component: extracellular space; plasma membrane. Molecular Function: hematopoietin/interferon-class (D200-domain) cytokine receptor activity; protein binding; interleukin-27 receptor binding; cytokine activity. Biological Process: T-helper 1 type immune response; cytokine and chemokine mediated signaling pathway; positive regulation of alpha-beta T cell proliferation; humoral immune response; positive regulation of interferon-gamma biosynthetic process

size1 :

48-Strip-Wells

price1 :

410 USD

size2 :

96-Strip-Wells

price2 :

490

more info or order :

MyBioSource product webpage