catalog number :
MBS2511984
products type :
ELISA Kit
products full name :
Human CA125 (Carbohydrate Antigen 125) ELISA Kit
products short name :
[CA125]
products gene name :
[CA125]
specificity :
This kit recognizes Human CA12 in samples. No significant cross-reactivity or interference between HumanCA12and analogues was observed.
storage stability :
Store at 4 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 0.31-20ng/mL. Sensitivity: 0.19ng/mL.
products description :
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human CA12 concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CA12. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CA12 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CA12, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of HumanCA12. You can calculate the concentration of Human CA12 in the samples by comparing the OD of the samples to the standard curve.
size5 :
10x96-Strip-Wells