ELISA/assay

Human pERK(Phospho Extracellular Signal Regulated Kinase)ELISA Kit

MBS2511875

48-Strip-Wells

410 USD

ELISA Kit

Human pERK(Phospho Extracellular Signal Regulated Kinase)ELISA Kit

pERK

PREDICTED: eukaryotic translation initiation factor 2-alpha kinase 3 isoform X1; Eukaryotic translation initiation factor 2-alpha kinase 3; eukaryotic translation initiation factor 2-alpha kinase 3; PRKR-like endoplasmic reticulum kinase; eukaryotic translation initiation factor 2 alpha kinase 3; hsPEK; pancreatic EIF2-alpha kinase; eukaryotic translation initiation factor 2-alpha kinase 3; PRKR-like endoplasmic reticulum kinase; Pancreatic eIF2-alpha kinase; HsPEK

pERK

EIF2AK3; EIF2AK3; PEK; WRS; PERK; PEK; PERK; HsPEK

E2AK3_HUMAN

Human

888

This kit recognizes natural and recombinant Human pERK. No significant cross-reactivity or interference between Human pERK and analogues was observed.

Store at 4 degree C.

Samples: Serum, plasma and other biological fluids. Assay Type: Sandwich. Detection Range: 31.25-2000pg/mL. Sensitivity: The minimum detectable dose of Human pERK is 18.75pg/mL

Intended Uses This ELISA kit applies to the in vitro quantitative determination of Human pERK concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to pERK. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for pERK and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain pERK, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of pERK. You can calculate the concentration of pERK in the samples by comparing the OD of the samples to the standard curve.

530368295

XP_005264706.1

XM_005264649.2

Q9NZJ5

125,216 Da

Alzheimer's Disease Pathway (83097); Alzheimer's Disease Pathway (509); Alzheimers Disease Pathway (672448); Epstein-Barr Virus Infection Pathway (585562); Epstein-Barr Virus Infection Pathway (587115); Hepatitis C Pathway (173973); Hepatitis C Pathway (173907); Herpes Simplex Infection Pathway (377873); Herpes Simplex Infection Pathway (377865); Influenza A Pathway (217173)

The protein encoded by this gene phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2 (EIF2), leading to its inactivation, and thus to a rapid reduction of translational initiation and repression of global protein synthesis. It is a type I membrane protein located in the endoplasmic reticulum (ER), where it is induced by ER stress caused by malfolded proteins. Mutations in this gene are associated with Wolcott-Rallison syndrome. [provided by RefSeq, Jan 2010]

PERK: a transmembrane protein kinase of the PEK family resident in the endoplasmic reticulum (ER) membrane and is linked to insulin processing. Couples ER stress to translation inhibition. Stress induces autophosphorylation of its kinase domain and increases its activity. Phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2 (eIF2alpha), leading to its inactivation and thus to a rapid reduction of translational initiation and repression of global protein synthesis. A critical effector of unfolded protein response (UPR)-induced G1 growth arrest due to the loss of cyclin d1. Forms dimers with BiP in resting cells. Oligomerizes in ER-stressed cells. LOF mutations cause Wolcott-Rallison syndrome (WRS), characterized by insulin-dependent diabetes in early infancy and, later, multiple system abnormalities. Neuronal death in Alzheimer?s and Parkinson?s diseases is thought to be due to ER stress and has been weakly linked to PEK. Protein type: Protein kinase, Other; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; EC 2.7.11.1; Translation; Membrane protein, integral; Other group; PEK family; PEK subfamily. Chromosomal Location of Human Ortholog: 2p12. Cellular Component: endoplasmic reticulum membrane; membrane; endoplasmic reticulum; cytoplasm; integral to endoplasmic reticulum membrane. Molecular Function: identical protein binding; protein serine/threonine kinase activity; protein binding; enzyme binding; protein phosphatase binding; eukaryotic translation initiation factor 2alpha kinase activity; ATP binding; protein kinase activity. Biological Process: lactation; fat cell differentiation; translation; positive regulation of protein binding; protein amino acid autophosphorylation; protein amino acid phosphorylation; bone mineralization; positive regulation of transcription from RNA polymerase I promoter; cellular response to glucose starvation; regulation of fatty acid metabolic process; angiogenesis; skeletal development; ER overload response; protein homooligomerization; endoplasmic reticulum organization and biogenesis; caspase activation; ossification; negative regulation of myelination; unfolded protein response; calcium-mediated signaling; chondrocyte development; SREBP-mediated signaling pathway; endocrine pancreas development; peptidyl-serine phosphorylation; cellular protein metabolic process; unfolded protein response, activation of signaling protein activity; insulin-like growth factor receptor signaling pathway; insulin secretion; negative regulation of translation; negative regulation of translation initiation in response to stress; negative regulation of apoptosis. Disease: Epiphyseal Dysplasia, Multiple, With Early-onset Diabetes Mellitus

48-Strip-Wells

410 USD

96-Strip-Wells

490