Rat GAL3(Galectin 3)ELISA Kit | MyBioSource MBS2511742 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rat GAL3(Galectin 3)ELISA Kit

catalog :

MBS2511742

quantity :

48-Strip-Wells

price :

410 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2511742

products type :

ELISA Kit

products full name :

Rat GAL3(Galectin 3)ELISA Kit

products short name :

GAL3

other names :

galectin-3 isoform 2; Galectin-3; galectin-3; 35 kDa lectin; IgE-binding protein; MAC-2 antigen; carbohydrate-binding protein 35; galactose-specific lectin 3; laminin-binding protein; lectin L-29; lectin, galactoside-binding, soluble, 3; 35 kDa lectin; Carbohydrate-binding protein 35; CBP 35; Galactose-specific lectin 3; Galactoside-binding protein; GALBP; IgE-binding protein; L-31; Laminin-binding protein; Lectin L-29; Mac-2 antigen

products gene name :

GAL3

other gene names :

LGALS3; LGALS3; L31; GAL3; MAC2; CBP35; GALBP; GALIG; LGALS2; MAC2; Gal-3; CBP 35; GALBP

uniprot entry name :

LEG3_HUMAN

reactivity :

Rat

sequence length :

200

specificity :

This kit recognizes natural and recombinant Rat GAL3. No significant cross-reactivity or interference between Rat GAL3 and analogues was observed.

storage stability :

Store at 4 degree C.

other info1 :

Samples: Serum, plasma and other biological fluids. Detection Range: 31.25-2000pg/mL. Sensitivity: The minimum detectable dose of Rat GAL3 is 18.75pg/mL (The sensitivity of this assay, or lowest detectable limit (LDL) was defined as the lowest protein concentration that could be differentiated from zero). Assay Type: Sandwich

other info2 :

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Rat GAL3 concentrations in serum, plasma and other biological fluids.

products description :

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to GAL3. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for GAL3 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain GAL3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of GAL3. You can calculate the concentration of GAL3 in the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

294345475

ncbi acc num :

NP_001170859.1

ncbi gb acc num :

NM_001177388.1

uniprot acc num :

P17931

ncbi mol weight :

26,152 Da

ncbi pathways :

AGE/RAGE Pathway (698754); Advanced Glycosylation Endproduct Receptor Signaling Pathway (187092); Hedgehog Signaling Events Mediated By Gli Proteins Pathway (137912); Immune System Pathway (106386); Innate Immune System Pathway (106387); Spinal Cord Injury Pathway (739007)

ncbi summary :

This gene encodes a member of the galectin family of carbohydrate binding proteins. Members of this protein family have an affinity for beta-galactosides. The encoded protein is characterized by an N-terminal proline-rich tandem repeat domain and a single C-terminal carbohydrate recognition domain. This protein can self-associate through the N-terminal domain allowing it to bind to multivalent saccharide ligands. This protein localizes to the extracellular matrix, the cytoplasm and the nucleus. This protein plays a role in numerous cellular functions including apoptosis, innate immunity, cell adhesion and T-cell regulation. The protein exhibits antimicrobial activity against bacteria and fungi. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Oct 2014]

uniprot summary :

Galectin-3: galactose-specific lectin which binds IgE. Expressed at a high level in the colonic epithelium. Also abundant in activated macrophages. Protein type: Extracellular matrix; Cell surface; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 14q22.3. Cellular Component: spliceosome; extracellular matrix; proteinaceous extracellular matrix; extracellular space; membrane; cytoplasm; mitochondrial inner membrane; plasma membrane; immunological synapse; nucleus; external side of plasma membrane. Molecular Function: protein binding; laminin binding; IgE binding; carbohydrate binding; chemoattractant activity. Biological Process: neutrophil chemotaxis; extracellular matrix organization and biogenesis; RNA splicing; negative regulation of endocytosis; regulation of T cell proliferation; negative regulation of T cell receptor signaling pathway; macrophage chemotaxis; monocyte chemotaxis; positive chemotaxis; epithelial cell differentiation; eosinophil chemotaxis; innate immune response; mRNA processing; skeletal development

size1 :

48-Strip-Wells

price1 :

410 USD

size2 :

96-Strip-Wells

price2 :

490

more info or order :

MyBioSource product webpage