catalog number :
MBS2511689
products type :
ELISA Kit
products full name :
Rat IL-1beta (Interleukin 1 Beta) ELISA Kit
products short name :
[IL-1beta]
other names :
[interleukin 1, beta; interleukin 1, beta; interleukin-1 beta]
products gene name :
[IL-1beta]
other gene names :
[IL1B2; IL1B; IL-1beta]
specificity :
This kit recognizes Rat IL-1ra/IL-1F3 in samples. No significant cross-reactivity or interference between Rat IL-1ra/IL-1F3 and analogues was observed.
storage stability :
Store at 4 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 78.13-5000pg/mL. Sensitivity: 46.88pg/mL.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat IL-1ra/IL-1F3 were tested 20 times on one plate, respectively. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat IL-1ra/IL-1F3 were tested on 3 different plates, 20 replicates in each plate.
products description :
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Rat IL-1ra/IL-1F3 concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat IL-1ra/IL-1F3. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat IL-1ra/IL-1F3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat IL-1ra/IL-1F3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Rat IL-1ra/IL-1F3. You can calculate the concentration of Rat IL-1ra/IL-1F3 in the samples by comparing the OD of the samples to the standard curve.
ncbi acc num :
NP_001005149.1
ncbi gb acc num :
NM_001005149.1
ncbi pathways :
African Trypanosomiasis Pathway (194379); African Trypanosomiasis Pathway (194323); Alzheimer's Disease Pathway (84508); Alzheimer's Disease Pathway (509); Amoebiasis Pathway (167313); Amoebiasis Pathway (167191); Apoptosis Pathway (84472); Apoptosis Pathway (470); Chagas Disease (American Trypanosomiasis) Pathway (147807); Chagas Disease (American Trypanosomiasis) Pathway (147795)
size5 :
10x96-Strip-Wells