ELISA/assay

Human S100A9 (S100 Calcium Binding Protein A9) ELISA Kit

MBS2511628

24-Strip-Wells

215 USD

ELISA Kit

Human S100A9 (S100 Calcium Binding Protein A9) ELISA Kit

[S100A9]

[S100A9, partial; Protein S100-A9; Calgranulin-B; Calprotectin L1H subunit; Leukocyte L1 complex heavy chain; Migration inhibitory factor-related protein 14; MRP-14; p14; S100 calcium-binding protein A9]

[S100A9]

[S100A9; CAGB; CFAG; MRP14; MRP-14; p14]

S10A9_HUMAN

Human

114

This kit recognizes natural and recombinant Human S100A9. No significant cross-reactivity or interference between Human S100A9 and analogues was observed.

Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 0.781-50ng/mL. Sensitivity: Min: 0.469ng/mL; Max: 50ng/mL

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human S100A9 concentrations in serum, plasma and other biological fluids.

Principle of the assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to S100A9. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for S100A9 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain S100A9, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of S100A9. You can calculate the concentration of S100A9 in the samples by comparing the OD of the samples to the standard curve.

49457442

CAG47020.1

P06702

13,242 Da

S100A9: a calcium-binding regulatory protein of the S-100 family expressed by macrophages in acutely inflammated tissues and in chronic inflammations. May be an inhibitor of protein kinases. Also expressed in epithelial cells constitutively or induced during dermatoses. May interact with components of the intermediate filaments in monocytes and epithelial cells. Interacts with CEACAM3 in a calcium-dependent manner. Protein type: Calcium-binding. Chromosomal Location of Human Ortholog: 1q21. Cellular Component: extracellular space; cytoskeleton; plasma membrane; extracellular region; nucleus; cytosol. Molecular Function: arachidonic acid binding; antioxidant activity; protein binding; RAGE receptor binding; signal transducer activity; zinc ion binding; microtubule binding; calcium ion binding. Biological Process: caspase activation; neutrophil chemotaxis; chronic inflammatory response; chemokine production; cytokine production; response to lipopolysaccharide; positive regulation of peptide secretion; signal transduction; positive regulation of cell growth; leukocyte migration during inflammatory response; activation of NF-kappaB transcription factor; sequestering of zinc ion; response to ethanol; cell-cell signaling; response to zinc ion; defense response to bacterium; actin cytoskeleton reorganization; autophagy; innate immune response; regulation of integrin biosynthetic process; inflammatory response; defense response to fungus; regulation of cytoskeleton organization and biogenesis; positive regulation of inflammatory response

24-Strip-Wells

215 USD

48-Strip-Wells

410

96-Strip-Wells

490

5x96-Strip-Wells

2040

10x96-Strip-Wells

3590