catalog number :
MBS2511621
products type :
ELISA Kit
products full name :
Mouse CK-MB (Creatine Kinase MB Isoenzyme) ELISA Kit
products short name :
[CK-MB]
other names :
[creatine kinase M-type; Creatine kinase M-type; creatine kinase M-type; creatine kinase M chain; creatine kinase, muscle; Creatine kinase M chain; M-CKCreatine kinase M-type, N-terminally processed]
products gene name :
[CK-MB]
other gene names :
[CKM; CKM; CKMM; M-CK; CKMM]
uniprot entry name :
KCRM_HUMAN
specificity :
This kit recognizes Mouse CCR8 in samples. No Significant cross-reactivity or interference between Mouse CCR8 and analogues was observed.
storage stability :
Store at 4 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 0.31-20 ng/mL. Sensitivity: 0.19 ng/mL
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse CCR8 were tested 20 times on one plate, respectively. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse CCR8 were tested on 3 different plates, 20 replicates in each plate.
products description :
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Mouse CCR8 concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse CCR8. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse CCR8 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CCR8, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse CCR8. You can calculate the concentration of Mouse CCR8 in the samples by comparing the OD of the samples to the standard curve.
ncbi acc num :
NP_001815.2
ncbi gb acc num :
NM_001824.4
ncbi mol weight :
43,101 Da
ncbi pathways :
Arginine And Proline Metabolism Pathway (82957); Arginine And Proline Metabolism Pathway (323); Creatine Metabolism Pathway (106192); Creatine Pathway (413359); Creatine Pathway (468234); Metabolic Pathways (132956); Metabolism Pathway (477135); Metabolism Of Amino Acids And Derivatives Pathway (106169); Regulation Of Retinoblastoma Protein Pathway (137916); Urea Cycle And Metabolism Of Amino Groups Pathway (198758)
ncbi summary :
The protein encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis and is an important serum marker for myocardial infarction. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in striated muscle as well as in other tissues, and as a heterodimer with a similar brain isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family. [provided by RefSeq, Jul 2008]
uniprot summary :
CKM: Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. Belongs to the ATP:guanido phosphotransferase family. Protein type: Amino Acid Metabolism - arginine and proline; EC 2.7.3.2; Kinase, other. Chromosomal Location of Human Ortholog: 19q13.32. Cellular Component: cytosol. Molecular Function: creatine kinase activity; ATP binding. Biological Process: phosphocreatine biosynthetic process; creatine metabolic process; phosphorylation
size5 :
10x96-Strip-Wells