Human EphA2 (Tyrosine protein kinase receptor A2) ELISA Kit | MyBioSource MBS2511581 product information

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product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human EphA2 (Tyrosine protein kinase receptor A2) ELISA Kit

catalog :

MBS2511581

quantity :

24-Strip-Wells

price :

215 USD

product information

catalog number :

MBS2511581

products type :

ELISA Kit

products full name :

Human EphA2 (Tyrosine protein kinase receptor A2) ELISA Kit

products short name :

[EphA2]

other names :

[ephrin type-A receptor 2; Ephrin type-A receptor 2; ephrin type-A receptor 2; epithelial cell receptor protein tyrosine kinase; soluble EPHA2 variant 1; tyrosine-protein kinase receptor ECK; EPH receptor A2; Epithelial cell kinase; Tyrosine-protein kinase receptor ECK]

products gene name :

[EphA2]

other gene names :

[EPHA2; EPHA2; ECK; CTPA; ARCC2; CTPP1; CTRCT6; ECK]

uniprot entry name :

EPHA2_HUMAN

reactivity :

Human

sequence length :

976

specificity :

This kit recognizes Human EFNA5 in samples. No significant cross-reactivity or interference between HumanEFNA5and analogues was observed.

storage stability :

Store at 4 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 0.16-10ng/mL. Sensitivity: 0.1ng/mL.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, midrange and high level HumanEFNA5were tested 20times on one plate, respectively. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, midrange and high level HumanEFNA5were testedon 3 different plates, 20 replicates in each plate.

products description :

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human EFNA5 concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human EFNA5. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human EFNA5 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human EFNA5, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human EFNA5. You can calculate the concentration of Human EFNA5 in the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

32967311

ncbi acc num :

NP_004422.2

ncbi gb acc num :

NM_004431.3

uniprot acc num :

P29317

ncbi mol weight :

54,305 Da

ncbi pathways :

Arf6 Signaling Events Pathway (138034); Axon Guidance Pathway (83065); Axon Guidance Pathway (476); Axon Guidance Pathway (105688); Developmental Biology Pathway (477129); Direct P53 Effectors Pathway (137939); EPH-Ephrin Signaling Pathway (1127691); EPH-ephrin Mediated Repulsion Of Cells Pathway (1127695); EPHA Forward Signaling Pathway (138041); EPHA-mediated Growth Cone Collapse Pathway (1127692)

ncbi summary :

This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene encodes a protein that binds ephrin-A ligands. Mutations in this gene are the cause of certain genetically-related cataract disorders.[provided by RefSeq, May 2010]

uniprot summary :

EphA2: a receptor tyrosine kinase. Receptor for members of the ephrin-A family. Binds to ephrin-A1, -A3, -A4 AND -A5. The Eph receptor tyrosine kinase family, the largest in the tyrosine kinase group, has fourteen members. They bind membrane-anchored ligands, ephrins, at sites of cell-cell contact, regulating the repulsion and adhesion of cells that underlie the establishment, maintenance, and remodeling of patterns of cellular organization. Eph signals are particularly important in regulating cell adhesion and cell migration during development, axon guidance, homeostasis and disease. EphA receptors bind to GPI-anchored ephrin-A ligands, while EphB receptors bind to ephrin-B proteins that have a transmembrane and cytoplasmic domain. Interactions between EphB receptor kinases and ephrin-B proteins transduce signals bidirectionally, signaling to both interacting cell types. Eph receptors and ephrins also regulate the adhesion of endothelial cells and are required for the remodeling of blood vessels. Overexpressed in many cancers including aggressive ovarian, cervical and breast carcinomas, and lung cancer. Expression correlates with degree of angiogenesis, metastasis and xenograft tumor growth. Soluble receptor inhibits tumor growth and angiogenesis in mice. Protein type: Kinase, protein; Protein kinase, tyrosine (receptor); Protein kinase, TK; EC 2.7.10.1; Membrane protein, integral; TK group; Eph family. Chromosomal Location of Human Ortholog: 1p36. Cellular Component: focal adhesion; cell surface; integral to plasma membrane; plasma membrane. Molecular Function: protein binding; ephrin receptor activity; transmembrane receptor protein tyrosine kinase activity; ATP binding. Biological Process: axial mesoderm formation; neural tube development; axon guidance; cell migration; negative regulation of protein kinase B signaling cascade; peptidyl-tyrosine phosphorylation; viral reproduction; multicellular organismal development; notochord formation; mammary gland epithelial cell proliferation; osteoclast differentiation; regulation of blood vessel endothelial cell migration; bone remodeling; regulation of angiogenesis; keratinocyte differentiation; osteoblast differentiation; regulation of cell adhesion mediated by integrin; DNA damage response, signal transduction resulting in induction of apoptosis; notochord cell development; protein kinase B signaling cascade; ephrin receptor signaling pathway; angiogenesis; vasculogenesis; cell adhesion; skeletal development. Disease: Cataract 6, Multiple Types

size1 :

24-Strip-Wells

price1 :

215 USD

size2 :

48-Strip-Wells

price2 :

410

size3 :

96-Strip-Wells

price3 :

490

size4 :

5x96-Strip-Wells

price4 :

2040

size5 :

10x96-Strip-Wells

price5 :

3590