Mouse BACE1 (Beta Site APP Cleaving Enzyme 1) ELISA Kit | MyBioSource MBS2511578 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse BACE1 (Beta Site APP Cleaving Enzyme 1) ELISA Kit

catalog :

MBS2511578

quantity :

24-Strip-Wells

price :

215 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS2511578

products type :

ELISA Kit

products full name :

Mouse BACE1 (Beta Site APP Cleaving Enzyme 1) ELISA Kit

products short name :

[BACE1]

other names :

[beta-secretase 1 isoform E; Beta-secretase 1; beta-secretase 1; APP beta-secretase; asp 2; aspartyl protease 2; beta-secretase 1 precursor variant 1; beta-site APP cleaving enzyme 1; beta-site amyloid beta A4 precursor protein-cleaving enzyme; memapsin-2; membrane-associated aspartic protease 2; transmembrane aspartic proteinase Asp2; beta-site APP-cleaving enzyme 1; Aspartyl protease 2; ASP2; Asp 2; Beta-site amyloid precursor protein cleaving enzyme 1; Beta-site APP cleaving enzyme 1; Memapsin-2; Membrane-associated aspartic protease 2]

products gene name :

[BACE1]

other gene names :

[BACE1; BACE1; ASP2; BACE; HSPC104; BACE; KIAA1149; ASP2; Asp 2; Beta-site APP cleaving enzyme 1]

uniprot entry name :

BACE1_HUMAN

reactivity :

Mouse

sequence length :

401

specificity :

This kit recognizes natural and recombinant Mouse BACE1. No significant cross-reactivity or interference between Mouse BACE1 and analogues was observed.

storage stability :

Store at 4 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 0.156-10ng/mL. Sensitivity: Min: 0.094ng/mL; Max: 10ng/mL

other info2 :

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Mouse BACE1 concentrations in serum, plasma and other biological fluids.

products description :

. Principle of the assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to BACE1. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for BACE1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain BACE1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of BACE1. You can calculate the concentration of BACE1 in the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

333440466

ncbi acc num :

NP_001193977.1

ncbi gb acc num :

NM_001207048.1

uniprot acc num :

P56817

ncbi mol weight :

42,107 Da

ncbi pathways :

Alzheimer's Disease Pathway (83097); Alzheimer's Disease Pathway (509); Alzheimers Disease Pathway (672448)

ncbi summary :

Cerebral deposition of amyloid beta peptide is an early and critical feature of Alzheimer's disease. Amyloid beta peptide is generated by proteolytic cleavage of amyloid precursor protein (APP) by two proteases, one of which is the protein encoded by this gene. The encoded protein, a member of the peptidase A1 protein family, is a type I integral membrane glycoprotein and aspartic protease that is found mainly in the Golgi. Multiple transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, May 2011]

uniprot summary :

BACE: an integral membrane glycoprotein and aspartic protease that is found mainly in the Golgi. Cleaves APP at the amino terminus of the A-beta peptide sequence, leading to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated carboxy-terminal fragment which is later release by gamma-secretase. Four splice-variant isoforms have been described. Protein type: Membrane protein, integral; Protease; EC 3.4.23.46. Chromosomal Location of Human Ortholog: 11q23.2-q23.3. Cellular Component: Golgi apparatus; multivesicular body; cell surface; cytoplasmic vesicle membrane; integral to plasma membrane; axon; endoplasmic reticulum; late endosome; integral to membrane; plasma membrane; trans-Golgi network; endosome. Molecular Function: protein binding; enzyme binding; beta-amyloid binding; beta-aspartyl-peptidase activity; aspartic-type endopeptidase activity. Biological Process: beta-amyloid metabolic process; membrane protein ectodomain proteolysis; proteolysis

size1 :

24-Strip-Wells

price1 :

215 USD

size2 :

48-Strip-Wells

price2 :

410

size3 :

96-Strip-Wells

price3 :

490

size4 :

5x96-Strip-Wells

price4 :

2040

size5 :

10x96-Strip-Wells

price5 :

3590

more info or order :

MyBioSource product webpage