catalog number :
MBS2511567
products type :
ELISA Kit
products full name :
Rat MMP-3 (Matrix Metalloproteinase 3) ELISA Kit
products short name :
MMP-3
other names :
MMP-3, partial; MMP-3; stromelysin-1; metalloproteinase 3 receptor; matrix metallopeptidase 3 (stromelysin 1, progelatinase)<; MMP-3
products gene name :
MMP-3
other gene names :
MMP3; bmmp-3
uniprot entry name :
Q9N283_BOVIN
specificity :
This kit recognizes natural and recombinant Rat MMP-3. No significant cross-reactivity or interference between Rat MMP-3 and analogues was observed.
storage stability :
Store at 4 degree C.
other info1 :
Samples: Serum, plasma and other biological fluids. Assay Type: Sandwich. Detection Range: 0.156-10ng/mL. Sensitivity: The minimum detectable dose of Rat MMP-3 is 0.094ng/mL (The sensitivity of this assay, or lowest detectable limit (LDL) was defined as the lowest protein concentration that could be differentiated from zero).
other info2 :
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Rat MMP-3 concentrations in serum, plasma and other biological fluids.
products description :
Principle of the assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to MMP-3. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for MMP-3 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain MMP-3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of MMP-3. You can calculate the concentration of MMP-3 in the samples by comparing the OD of the samples to the standard curve.
ncbi acc num :
BAA96388.1
ncbi mol weight :
16,673 Da
ncbi pathways :
Matrix Metalloproteinases Pathway (198565); Rheumatoid Arthritis Pathway (200308); Rheumatoid Arthritis Pathway (200269); TNF Signaling Pathway (812258); TNF Signaling Pathway (813210); Transcriptional Misregulation In Cancer Pathway (522997); Transcriptional Misregulation In Cancer Pathway (522987)