Rat VE-Cadherin (Vascular Endothelial Cadherin) ELISA Kit | MyBioSource MBS2511473 product information

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product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rat VE-Cadherin (Vascular Endothelial Cadherin) ELISA Kit

catalog :

MBS2511473

quantity :

24-Strip-Wells

price :

190 USD

product information

catalog number :

MBS2511473

products type :

ELISA Kit

products full name :

Rat VE-Cadherin (Vascular Endothelial Cadherin) ELISA Kit

products short name :

[VE-Cadherin]

other names :

[VE-cadherin; Cadherin-5; cadherin-5; 7B4 antigen; VE-cadherin; cadherin 5, type 2, VE-cadherin (vascular epithelium); cd144 antigen; endothelial-specific cadherin; vascular endothelial cadherin; cadherin 5, type 2 (vascular endothelium); 7B4 antigen; Vascular endothelial cadherin; VE-cadherin; CD_antigen: CD144]

products gene name :

[VE-Cadherin]

other gene names :

[CDH5; CDH5; 7B4; CD144; VE-cadherin]

uniprot entry name :

CADH5_HUMAN

reactivity :

Rat

sequence length :

784

specificity :

This kit recognizes Rat VCAM-1/CD106 in samples. No significant cross-reactivity or interference between Rat VCAM-1/CD106and analogues was observed.

storage stability :

Store at 4 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 12.5-800pg/mL. Sensitivity: 7.5pg/mL.

products description :

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Rat VCAM-1/CD106concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat VCAM-1/CD106. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat VCAM-1/CD106 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat VCAM-1/CD106, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Rat VCAM-1/CD106. You can calculate the concentration of Rat VCAM-1/CD106in the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

599834

ncbi acc num :

CAA56306.1

uniprot acc num :

P33151

ncbi mol weight :

74,499 Da

ncbi pathways :

Adherens Junctions Interactions Pathway (119533); Cell Adhesion Molecules (CAMs) Pathway (83069); Cell Adhesion Molecules (CAMs) Pathway (480); Cell Junction Organization Pathway (160966); Cell-Cell Communication Pathway (477132); Cell-cell Junction Organization Pathway (119532); Leukocyte Transendothelial Migration Pathway (83083); Leukocyte Transendothelial Migration Pathway (494); S1P2 Pathway (138059); Signal Transduction Pathway (477114)

ncbi summary :

This gene is a classical cadherin from the cadherin superfamily and is located in a six-cadherin cluster in a region on the long arm of chromosome 16 that is involved in loss of heterozygosity events in breast and prostate cancer. The encoded protein is a calcium-dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Functioning as a classic cadherin by imparting to cells the ability to adhere in a homophilic manner, the protein may play an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. An alternative splice variant has been described but its full length sequence has not been determined. [provided by RefSeq, Jul 2008]

uniprot summary :

CDH5: Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. This cadherin may play a important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. It associates with alpha-catenin forming a link to the cytoskeleton. Acts in concert with KRIT1 to establish and maintain correct endothelial cell polarity and vascular lumen. These effects are mediated by recruitment and activation of the Par polarity complex and RAP1B. Required for activation of PRKCZ and for the localization of phosphorylated PRKCZ, PARD3, TIAM1 and RAP1B to the cell junction. Interacts via cadherin 5 domain with PTPRB. Interacts with TRPC4. Interacts with KRIT1. Endothelial tissues and brain. Protein type: Motility/polarity/chemotaxis; Cell adhesion; Membrane protein, integral. Chromosomal Location of Human Ortholog: 16q22.1. Cellular Component: adherens junction; tight junction; membrane; plasma membrane; integral to membrane; intercellular junction; cell junction. Molecular Function: protein binding; beta-catenin binding; calcium ion binding; protein phosphatase binding; receptor binding. Biological Process: intercellular junction assembly and maintenance; negative regulation of cell proliferation; homophilic cell adhesion; vascular endothelial growth factor receptor signaling pathway; blood vessel maturation

size1 :

24-Strip-Wells

price1 :

190 USD

size2 :

48-Strip-Wells

price2 :

310

size3 :

96-Strip-Wells

price3 :

355

size4 :

5x96-Strip-Wells

price4 :

1435

size5 :

10x96-Strip-Wells

price5 :

2805