ELISA/assay

Mouse PAPPA(Pregnancy Associated Plasma Protein A)ELISA Kit

MBS2511453

48-Strip-Wells

410 USD

ELISA Kit

Mouse PAPPA(Pregnancy Associated Plasma Protein A)ELISA Kit

PAPPA

pappalysin-1 preproprotein; Pappalysin-1; pappalysin-1; IGF-dependent IGFBP-4 protease; aspecific BCL2 ARE-binding protein 2; differentially placenta 1 expressed protein; insulin-like growth factor-dependent IGF binding protein-4 protease; insulin-like growth factor-dependent IGF-binding protein 4 protease; pregnacy-associated plasma protein A; pregnancy-associated plasma protein A, pappalysin 1; Insulin-like growth factor-dependent IGF-binding protein 4 protease; IGF-dependent IGFBP-4 protease; IGFBP-4ase; Pregnancy-associated plasma protein A; PAPP-A

PAPPA

PAPPA; PAPPA; PAPA; DIPLA1; PAPP-A; PAPPA1; ASBABP2; IGFBP-4ase; IGF-dependent IGFBP-4 protease; IGFBP-4ase; PAPP-A

PAPP1_HUMAN

Mouse

1627

This assay has high sensitivity and excellent specificity for detection of Mouse PAPPA. No significant cross-reactivity or interference between Mouse PAPPA and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Mouse PAPPA and all the analogues, therefore, cross reaction may still exist.

Store at 4 degree C.

Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 0.156--10ng/mL. Sensitivity: 0.094ng/mL

Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of PAPPA in Mouse serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse PAPPA. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Mouse PAPPA and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse PAPPA, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse PAPPA. You can calculate the concentration of Mouse PAPPA in the samples by comparing the OD of the samples to the standard curve.

38045915

NP_002572.2

NM_002581.3

Q13219

180,973 Da

Metabolism Of Proteins Pathway (106230); Regulation Of Insulin-like Growth Factor (IGF) Transport And Uptake By Insulin-like Growth Factor Binding Proteins (IGFBPs) Pathway (105908)

This gene encodes a secreted metalloproteinase which cleaves insulin-like growth factor binding proteins (IGFBPs). It is thought to be involved in local proliferative processes such as wound healing and bone remodeling. Low plasma level of this protein has been suggested as a biochemical marker for pregnancies with aneuploid fetuses. [provided by RefSeq, Jul 2008]

PAPPA: Metalloproteinase which specifically cleaves IGFBP-4 and IGFBP-5, resulting in release of bound IGF. Cleavage of IGFBP-4 is dramatically enhanced by the presence of IGF, whereas cleavage of IGFBP-5 is slightly inhibited by the presence of IGF. Belongs to the peptidase M43B family. Protein type: Protease; Secreted; EC 3.4.24.79; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 9q33.2. Cellular Component: membrane; extracellular region. Molecular Function: metallopeptidase activity; zinc ion binding; metalloendopeptidase activity. Biological Process: cellular protein metabolic process; female pregnancy; proteolysis; cell differentiation

48-Strip-Wells

410 USD

96-Strip-Wells

490