cAMP (Cyclic adenosine monophosphate) ELISA Kit | MyBioSource MBS2511445 product information

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product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

cAMP (Cyclic adenosine monophosphate) ELISA Kit

catalog :

MBS2511445

quantity :

24-Strip-Wells

price :

190 USD

product information

catalog number :

MBS2511445

products type :

ELISA Kit

products full name :

cAMP (Cyclic adenosine monophosphate) ELISA Kit

products short name :

[cAMP]

other names :

[chromosome alignment-maintaining phosphoprotein 1; Chromosome alignment-maintaining phosphoprotein 1; chromosome alignment-maintaining phosphoprotein 1; zinc finger protein 828; chromosome alignment maintaining phosphoprotein 1; Zinc finger protein 828]

products gene name :

[cAMP]

other gene names :

[CHAMP1; CHAMP1; CAMP; CHAMP; ZNF828; C13orf8; C13orf8; CAMP; KIAA1802; ZNF828]

uniprot entry name :

CHAP1_HUMAN

reactivity :

General

sequence length :

812

specificity :

This kit recognizes Bovine, Horse, Sheep E2 in samples. No significant cross-reactivity or interference between E2 and analogues was observed.

storage stability :

Store at 4 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum And Plasma Samples. Assay Type: Quantitative Competitive. Detection Range: 9.37-600pg/mL. Sensitivity: 5.63pg/mL.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level E2 were tested 20 times on one plate, respectively. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level E2 were tested on 3 different plates, 20 replicates in each plate.

products description :

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Bovine, Horse, Sheep E2 concentrations in serum and plasma samples. Please contact tech-support for other sample type detection. Principle of the Assay: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with E2. During the reaction, E2 in the sample or standard competes with a fixed amount of E2 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to E2. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of E2 in the samples is then determined by comparing the OD of the samples to the standard curve!!Background/Introduction: Estradiol is a female sex hormone produced by the ovaries, adrenal gland and also the placenta during pregnancy. Estradiol is the most important hormone during a female's reproductive years, and is required for reproductive and sexual function as well as having an impact on the health of other organs and tissues. In females, estradiol acts primarily as a growth hormone for the reproductive organs including the vagina, the fallopian tubes, the endometrium and the cervical glands [1]. Estradiol also enhances growth of the womb's muscle layer, the myometrium. In addition, the hormone maintains oocytes and triggers a series of events that lead to ovulation. Though estradiol levels in men are much lower compared to those in women, estradiol has important roles in men as well. Aside from humans and other mammals, estradiol is also found in most vertebrates and crustaceans, insects, fish, and other animal species. Estradiol is produced especially within the follicles of the ovaries, but also in other tissues including the testicles, the adrenal glands, fat, liver, the breasts, and the brain. Estradiol is produced in the body from cholesterol through a series of reactions and intermediates [2].

ncbi gi num :

255918139

ncbi acc num :

NP_001157616.1

ncbi gb acc num :

NM_001164144.1

uniprot acc num :

Q96JM3

ncbi mol weight :

89,099 Da

uniprot summary :

ZNF828: Required for proper alignment of chromosomes at metaphase and their accurate segregation during mitosis. Involved in the maintenance of spindle microtubules attachment to the kinetochore during sister chromatid biorientation. May recruit CENPE and CENPF to the kinetochore. Protein type: C2H2-type zinc finger protein; RNA-binding. Chromosomal Location of Human Ortholog: 13q34. Cellular Component: nucleoplasm; cytoplasm; spindle; condensed chromosome; nucleus. Molecular Function: protein binding; nucleic acid binding; metal ion binding. Biological Process: attachment of spindle microtubules to kinetochore during mitosis; sister chromatid biorientation

size1 :

24-Strip-Wells

price1 :

190 USD

size2 :

48-Strip-Wells

price2 :

310

size3 :

96-Strip-Wells

price3 :

355

size4 :

5x96-Strip-Wells

price4 :

1435

size5 :

10x96-Strip-Wells

price5 :

2805