Human ANG2 (Angiopoietin 2) ELISA Kit | MyBioSource MBS2511414 product information

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product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human ANG2 (Angiopoietin 2) ELISA Kit

catalog :

MBS2511414

quantity :

24-Strip-Wells

price :

190 USD

product information

catalog number :

MBS2511414

products type :

ELISA Kit

products full name :

Human ANG2 (Angiopoietin 2) ELISA Kit

products short name :

[ANG2]

other names :

[angiopoietin-2 isoform c; Angiopoietin-2; angiopoietin-2; ANG-2; Tie2-ligand; angiopoietin-2B; angiopoietin-2a; angiopoietin 2]

products gene name :

[ANG2]

other gene names :

[ANGPT2; ANGPT2; ANG2; AGPT2; ANG-2]

uniprot entry name :

ANGP2_HUMAN

reactivity :

Human

sequence length :

444

specificity :

This kit recognizes natural and recombinantHumanANG2. No significant cross-reactivity or interference between HumanANG2 and analogues was observed.

storage stability :

Store at 4 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 46.875-3000pg/mL. Sensitivity: Min: 28.125pg/mL; Max: 3000pg/mL

products description :

Intended Uses: This ELISA kit applies to the invitro quantitative determination of HumanANG2concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to ANG2. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for ANG2and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain ANG2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of ANG2.You can calculate the concentration of ANG2in the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

169646754

ncbi acc num :

NP_001112360.1

ncbi gb acc num :

NM_001118888.1

uniprot acc num :

O15123

ncbi mol weight :

56,848 Da

ncbi pathways :

Angiopoietin Receptor Tie2-mediated Signaling Pathway (137917); Cell Surface Interactions At The Vascular Wall Pathway (106062); HIF-1 Signaling Pathway (695200); Hemostasis Pathway (106028); PI3K-Akt Signaling Pathway (692234); PI3K-Akt Signaling Pathway (692979); Rap1 Signaling Pathway (868086); Rap1 Signaling Pathway (878042); Ras Signaling Pathway (868085); Tie2 Signaling Pathway (106063)

ncbi summary :

The protein encoded by this gene is an antagonist of angiopoietin 1 (ANGPT1) and endothelial TEK tyrosine kinase (TIE-2, TEK). The encoded protein disrupts the vascular remodeling ability of ANGPT1 and may induce endothelial cell apoptosis. Three transcript variants encoding three different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

uniprot summary :

ANGPT2: Binds to TEK/TIE2, competing for the ANGPT1 binding site, and modulating ANGPT1 signaling. Can induce tyrosine phosphorylation of TEK/TIE2 in the absence of ANGPT1. In the absence of angiogenic inducers, such as VEGF, ANGPT2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal. Interacts with TEK/TIE2, competing for the same binding site as ANGPT1. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 8p23.1. Cellular Component: extracellular space; cell projection; extracellular region; plasma membrane; nucleus. Molecular Function: protein binding; metal ion binding; receptor tyrosine kinase binding; receptor binding. Biological Process: organ regeneration; Tie receptor signaling pathway; maternal process involved in pregnancy; signal transduction; negative regulation of blood vessel endothelial cell migration; response to organic cyclic substance; germ cell development; negative regulation of positive chemotaxis; positive regulation of angiogenesis; negative regulation of angiogenesis; response to radiation; response to mechanical stimulus; response to hypoxia; response to glucose stimulus; response to activity; angiogenesis; blood coagulation; leukocyte migration

size1 :

24-Strip-Wells

price1 :

190 USD

size2 :

48-Strip-Wells

price2 :

310

size3 :

96-Strip-Wells

price3 :

355

size4 :

5x96-Strip-Wells

price4 :

1435

size5 :

10x96-Strip-Wells

price5 :

2805