Human CA1 (Carbonic Anhydrase I) ELISA Kit | MyBioSource MBS2511363 product information

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product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human CA1 (Carbonic Anhydrase I) ELISA Kit

catalog :

MBS2511363

quantity :

24-Strip-Wells

price :

215 USD

product information

catalog number :

MBS2511363

products type :

ELISA Kit

products full name :

Human CA1 (Carbonic Anhydrase I) ELISA Kit

products short name :

[CA1]

other names :

[carbonic anhydrase 1 isoform a; Carbonic anhydrase 1; carbonic anhydrase 1; carbonate dehydratase I; carbonic anhydrase B; epididymis secretory protein Li 11; carbonic anhydrase I; Carbonate dehydratase I; Carbonic anhydrase B; CAB; Carbonic anhydrase I; CA-I]

products gene name :

[CA1]

other gene names :

[CA1; CA1; CAB; CA-I; Car1; HEL-S-11; CAB; CA-I]

uniprot entry name :

CAH1_HUMAN

reactivity :

Human

sequence length :

261

specificity :

This kit recognizes Human C1q in samples. No Significant cross-reactivity or interference between Human C1q and analogues was observed.

storage stability :

Store at 4 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 78.13-5000 pg/mL. Sensitivity: 46.88 pg/mL

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human C1q were tested 20 times on one plate, respectively. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human C1q were tested on 3 different plates, 20 replicates in each plate.

products description :

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human C1q concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human C1q. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human C1q and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human C1q, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human C1q. You can calculate the concentration of Human C1q in the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

192447434

ncbi acc num :

NP_001122303.1

ncbi gb acc num :

NM_001128831.3

uniprot acc num :

P00915

ncbi mol weight :

28,870 Da

ncbi pathways :

C-MYB Transcription Factor Network Pathway (138073); Erythrocytes Take Up Carbon Dioxide And Release Oxygen Pathway (645347); Erythrocytes Take Up Oxygen And Release Carbon Dioxide Pathway (645348); Metabolism Pathway (477135); Nitrogen Metabolism Pathway (83025); Nitrogen Metabolism Pathway (416); O2/CO2 Exchange In Erythrocytes Pathway (645346); Reversible Hydration Of Carbon Dioxide Pathway (645352)

ncbi summary :

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. This CA1 gene is closely linked to the CA2 and CA3 genes on chromosome 8. It encodes a cytosolic protein that is found at the highest level in erythrocytes. Allelic variants of this gene have been described in some populations. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]

uniprot summary :

CA1: Reversible hydration of carbon dioxide. Can hydrates cyanamide to urea. Belongs to the alpha-carbonic anhydrase family. Protein type: Lyase; Energy Metabolism - nitrogen; EC 4.2.1.1. Chromosomal Location of Human Ortholog: 8q21.2. Cellular Component: cytosol. Molecular Function: carbonate dehydratase activity; zinc ion binding. Biological Process: bicarbonate transport; one-carbon compound metabolic process

size1 :

24-Strip-Wells

price1 :

215 USD

size2 :

48-Strip-Wells

price2 :

410

size3 :

96-Strip-Wells

price3 :

490

size4 :

5x96-Strip-Wells

price4 :

2040

size5 :

10x96-Strip-Wells

price5 :

3590