Human GPC1 (Glypican 1) ELISA Kit | MyBioSource MBS2511357 product information

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product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human GPC1 (Glypican 1) ELISA Kit

catalog :

MBS2511357

quantity :

24-Strip-Wells

price :

215 USD

product information

catalog number :

MBS2511357

products type :

ELISA Kit

products full name :

Human GPC1 (Glypican 1) ELISA Kit

products short name :

[GPC1]

other names :

[glypican-1; Glypican-1; glypican-1; glypican proteoglycan 1; glypican 1]

products gene name :

[GPC1]

other gene names :

[GPC1; GPC1; glypican]

uniprot entry name :

GPC1_HUMAN

reactivity :

Human

sequence length :

558

specificity :

This kit recognizes natural and recombinant Human GPC1. No significant cross-reactivity or interference between Human GPC1 and analogues was observed.

storage stability :

Store at 4 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 78.125-5000pg/mL. Sensitivity: Min: 46.875pg/mL; Max: 5000pg/mL

other info2 :

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human GPC1 concentrations in serum, plasma and other biological fluids.

products description :

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to GPC1. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for GPC1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain GPC1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of GPC1. You can calculate the concentration of GPC1 in the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

167001141

ncbi acc num :

NP_002072.2

ncbi gb acc num :

NM_002081.2

uniprot acc num :

P35052

ncbi mol weight :

31,931 Da

ncbi pathways :

A Tetrasaccharide Linker Sequence Is Required For GAG Synthesis Pathway (645305); Activation Of Rac Pathway (119530); Axon Guidance Pathway (105688); Chondroitin Sulfate/dermatan Sulfate Metabolism Pathway (645308); Defective B3GAT3 Causes JDSSDHD Pathway (1127588); Defective B4GALT1 Causes B4GALT1-CDG (CDG-2d) Pathway (1127592); Defective B4GALT7 Causes EDS, Progeroid Type Pathway (1127587); Defective CHST14 Causes EDS, Musculocontractural Type Pathway (1127591); Defective CHST3 Causes SEDCJD Pathway (1127590); Defective CHST6 Causes MCDC1 Pathway (1127593)

ncbi summary :

Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. [provided by RefSeq, Jul 2008]

uniprot summary :

GPC1: Cell surface proteoglycan that bears heparan sulfate. Binds, via the heparan sulfate side chains, alpha-4 (V) collagen and participates in Schwann cell myelination. May act as a catalyst in increasing the rate of conversion of prion protein PRPN(C) to PRNP(Sc) via associating (via the heparan sulfate side chains) with both forms of PRPN, targeting them to lipid rafts and facilitating their interaction. Required for proper skeletal muscle differentiation by sequestering FGF2 in lipid rafts preventing its binding to receptors (FGFRs) and inhibiting the FGF-mediated signaling. Associates (via the heparan sulfate side chains) with fibrillar APP-beta amyloid peptides in primitive and classic amyloid plaques and may be involved in the deposition of these senile plaques in the Alzheimer disease (AD) brain. Misprocessing of GPC1 is found in fibroblasts of patients with Niemann-Pick Type C1 disease. This is due to the defective deaminative degradation of heparan sulfate chains. Belongs to the glypican family. Protein type: Extracellular matrix; Membrane protein, GPI anchor; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 2q35-q37. Cellular Component: extracellular space; proteinaceous extracellular matrix; lysosomal lumen; Golgi lumen; integral to plasma membrane; plasma membrane; endosome; lipid raft. Molecular Function: heparan sulfate proteoglycan binding; fibroblast growth factor binding; copper ion binding; laminin binding. Biological Process: axon guidance; phototransduction, visible light; heparan sulfate proteoglycan catabolic process; glycosaminoglycan metabolic process; negative regulation of fibroblast growth factor receptor signaling pathway; pathogenesis; Schwann cell differentiation; chondroitin sulfate metabolic process; myelin formation; glycosaminoglycan biosynthetic process; glycosaminoglycan catabolic process; carbohydrate metabolic process; retinoid metabolic process

size1 :

24-Strip-Wells

price1 :

215 USD

size2 :

48-Strip-Wells

price2 :

410

size3 :

96-Strip-Wells

price3 :

490

size4 :

5x96-Strip-Wells

price4 :

2040

size5 :

10x96-Strip-Wells

price5 :

3590