Rat HDAC2 (Histone Deacetylase 2) ELISA Kit | MyBioSource MBS2511276 product information

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product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rat HDAC2 (Histone Deacetylase 2) ELISA Kit

catalog :

MBS2511276

quantity :

24-Strip-Wells

price :

215 USD

product information

catalog number :

MBS2511276

products type :

ELISA Kit

products full name :

Rat HDAC2 (Histone Deacetylase 2) ELISA Kit

products short name :

[HDAC2]

other names :

[histone deacetylase 2; Histone deacetylase 2; histone deacetylase 2; YY1-associated factor 1; transcriptional regulator homolog RPD3; histone deacetylase 2]

products gene name :

[HDAC2]

other gene names :

[HDAC2; HDAC2; HD2; RPD3; YAF1; HD2]

uniprot entry name :

HDAC2_HUMAN

reactivity :

Rat

sequence length :

488

specificity :

This kit recognizes Rat HbA1c in samples. No significant cross-reactivity or interference between Rat HbA1c and analogues was observed.

storage stability :

Store at 4 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 1.56-100ng/mL. Sensitivity: 0.94ng/mL.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat HbA1c were tested 20 times on one plate, respectively. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat HbA1c were tested on 3 different plates, 20 replicates in each plate.

products description :

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Rat HbA1c concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat HbA1c. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat HbA1c and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat HbA1c, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Rat HbA1c. You can calculate the concentration of Rat HbA1c in the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

293336691

ncbi acc num :

NP_001518.3

ncbi gb acc num :

NM_001527.3

uniprot acc num :

Q92769

ncbi mol weight :

51,998 Da

ncbi pathways :

Alcoholism Pathway (585563); Alcoholism Pathway (587116); Cell Cycle Pathway (198811); Cell Cycle Pathway (83054); Cell Cycle Pathway (463); Chromatin Modifying Enzymes Pathway (1127682); Chromatin Organization Pathway (1127681); Chronic Myeloid Leukemia Pathway (83116); Chronic Myeloid Leukemia Pathway (528); Constitutive Signaling By NOTCH1 HD+PEST Domain Mutants Pathway (771595)

ncbi summary :

This gene product belongs to the histone deacetylase family. Histone deacetylases act via the formation of large multiprotein complexes, and are responsible for the deacetylation of lysine residues at the N-terminal regions of core histones (H2A, H2B, H3 and H4). This protein forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. Thus, it plays an important role in transcriptional regulation, cell cycle progression and developmental events. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2010]

uniprot summary :

HDAC2: a transcriptional regulator of the histone deacetylase family, subfamily 1. Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation plays a role in epigenetic repression and transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Protein type: EC 3.5.1.98; Deacetylase; Transcription, coactivator/corepressor; Nuclear receptor co-regulator. Chromosomal Location of Human Ortholog: 6q21. Cellular Component: nucleoplasm; heterochromatin; transcription factor complex; protein complex; Sin3 complex; ESC/E(Z) complex; nuclear chromatin; cytoplasm; replication fork; NuRD complex; nucleus. Molecular Function: deacetylase activity; protein binding; NAD-dependent histone deacetylase activity (H3-K9 specific); enzyme binding; nucleosomal DNA binding; NAD-dependent histone deacetylase activity (H3-K14 specific); sequence-specific DNA binding; heat shock protein binding; protein deacetylase activity; NAD-dependent histone deacetylase activity (H4-K16 specific); chromatin binding; histone deacetylase activity; transcription factor binding; transcription factor activity. Biological Process: establishment and/or maintenance of chromatin architecture; nerve growth factor receptor signaling pathway; positive regulation of proteolysis; positive regulation of transcription, DNA-dependent; positive regulation of collagen biosynthetic process; negative regulation of transcription from RNA polymerase II promoter; regulation of gene expression, epigenetic; negative regulation of cell cycle; cardiac muscle cell development; negative regulation of cardiac muscle cell proliferation; negative regulation of gene expression, epigenetic; positive regulation of cell proliferation; dendrite development; circadian regulation of gene expression; positive regulation of oligodendrocyte differentiation; response to drug; transcription, DNA-dependent; negative regulation of transcription factor activity; hippocampus development; histone deacetylation; regulation of protein kinase B signaling cascade; response to cocaine; odontogenesis of dentine-containing teeth; chromatin remodeling; negative regulation of MHC class II biosynthetic process; maintenance of chromatin silencing; ATP-dependent chromatin remodeling; epidermal cell differentiation; gene expression; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; embryonic digit morphogenesis; blood coagulation; negative regulation of transcription, DNA-dependent; negative regulation of apoptosis

size1 :

24-Strip-Wells

price1 :

215 USD

size2 :

48-Strip-Wells

price2 :

410

size3 :

96-Strip-Wells

price3 :

490

size4 :

5x96-Strip-Wells

price4 :

2040

size5 :

10x96-Strip-Wells

price5 :

3590