Rat GIP (Gastric Inhibitory Polypeptide) ELISA Kit | MyBioSource MBS2511191 product information

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product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rat GIP (Gastric Inhibitory Polypeptide) ELISA Kit

catalog :

MBS2511191

quantity :

24-Strip-Wells

price :

215 USD

product information

catalog number :

MBS2511191

products type :

ELISA Kit

products full name :

Rat GIP (Gastric Inhibitory Polypeptide) ELISA Kit

products short name :

[GIP]

other names :

[gastric inhibitory polypeptide preproprotein; Gastric inhibitory polypeptide; gastric inhibitory polypeptide; glucose-dependent insulinotropic polypeptide; incretin hormone; gastric inhibitory polypeptide; Glucose-dependent insulinotropic polypeptide; Incretin hormone]

products gene name :

[GIP]

other gene names :

[GIP; GIP; GIP]

uniprot entry name :

GIP_HUMAN

reactivity :

Rat

sequence length :

153

specificity :

This kit recognizes natural and recombinant Rat GIP. No significant cross-reactivity or interference between Rat GIP and analogues was observed.

storage stability :

Store at 4 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 0.625-40ng/mL. Sensitivity: Min: 0.375ng/mL; Max: 40ng/mL

other info2 :

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Rat GIP concentrations in serum, plasma and other biological fluids.

products description :

Principle of the assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to GIP. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for GIP and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain GIP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of GIP. You can calculate the concentration of GIP in the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

4758436

ncbi acc num :

NP_004114.1

ncbi gb acc num :

NM_004123.2

uniprot acc num :

P09681

ncbi mol weight :

17,108 Da

ncbi pathways :

Class B/2 (Secretin Family Receptors) Pathway (106378); Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway (1127664); Disease Pathway (530764); G Alpha (s) Signalling Events Pathway (119549); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020); Glucagon-type Ligand Receptors Pathway (106380); Incretin Synthesis, Secretion, And Inactivation Pathway (187170); Insulin Secretion Pathway (777534); Metabolic Disorders Of Biological Oxidation Enzymes Pathway (1127637)

ncbi summary :

This gene encodes an incretin hormone and belongs to the glucagon superfamily. The encoded protein is important in maintaining glucose homeostasis as it is a potent stimulator of insulin secretion from pancreatic beta-cells following food ingestion and nutrient absorption. This gene stimulates insulin secretion via its G protein-coupled receptor activation of adenylyl cyclase and other signal transduction pathways. It is a relatively poor inhibitor of gastric acid secretion. [provided by RefSeq, Jul 2008]

uniprot summary :

GIP: Potent stimulator of insulin secretion and relatively poor inhibitor of gastric acid secretion. Belongs to the glucagon family. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 17q21.3-q22. Cellular Component: extracellular space; cell soma; endoplasmic reticulum lumen; extracellular region. Molecular Function: hormone activity. Biological Process: response to drug; response to peptide hormone stimulus; adult locomotory behavior; positive regulation of insulin secretion; female pregnancy; sensory perception of pain; response to amino acid stimulus; response to lipid; signal transduction; endocrine pancreas development; response to organic cyclic substance; memory; response to starvation; response to selenium ion; cellular protein metabolic process; response to glucose stimulus; response to axon injury; regulation of insulin secretion

size1 :

24-Strip-Wells

price1 :

215 USD

size2 :

48-Strip-Wells

price2 :

410

size3 :

96-Strip-Wells

price3 :

490

size4 :

5x96-Strip-Wells

price4 :

2040

size5 :

10x96-Strip-Wells

price5 :

3590