Human GSTpi (Glutathione S Transferases Pi) ELISA Kit | MyBioSource MBS2511042 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human GSTpi (Glutathione S Transferases Pi) ELISA Kit

catalog :

MBS2511042

quantity :

24-Strip-Wells

price :

215 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS2511042

products type :

ELISA Kit

products full name :

Human GSTpi (Glutathione S Transferases Pi) ELISA Kit

products short name :

[GSTpi]

other names :

[glutathione S-transferase-P1c; Glutathione S-transferase P; glutathione S-transferase P; GST class-pi; GSTP1-1; deafness, X-linked 7; epididymis secretory protein Li 22; fatty acid ethyl ester synthase III; glutathione S-transferase pi 1; GST class-pi; GSTP1-1]

products gene name :

[GSTpi]

other gene names :

[GSTP1; GSTP1; PI; DFN7; GST3; GSTP; FAEES3; HEL-S-22; FAEES3; GST3]

uniprot entry name :

GSTP1_HUMAN

reactivity :

Human

sequence length :

210

specificity :

This kit recognizes natural and recombinantHumanGSTpi. No significant cross-reactivity or interference between HumanGSTpi and analogues was observed.

storage stability :

Store at 4 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 2.344-150ng/mL. Sensitivity: Min: 1.406ng/mL; Max: 150ng/mL

products description :

Intended Uses: This ELISA kit applies to the invitro quantitative determination of HumanGSTpiconcentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to GSTpi. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for GSTpiand Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain GSTpi, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of GSTpi.You can calculate the concentration of GSTpiin the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

726098

ncbi acc num :

AAC13869.1

uniprot acc num :

P09211

ncbi mol weight :

23,356 Da

ncbi pathways :

4-hydroxy-2-nonenal Detoxification Pathway (703096); Arachidonate Epoxygenase / Epoxide Hydrolase Pathway (198838); Biological Oxidations Pathway (105698); Cellular Responses To Stress Pathway (645258); Chemical Carcinogenesis Pathway (673221); Chemical Carcinogenesis Pathway (673237); Defective AHCY Causes Hypermethioninemia With S-adenosylhomocysteine Hydrolase Deficiency (HMAHCHD) Pathway (1127639); Defective GCLC Causes Hemolytic Anemia Due To Gamma-glutamylcysteine Synthetase Deficiency (HAGGSD) Pathway (1127658); Defective GGT1 Causes Glutathionuria (GLUTH) Pathway (1127659); Defective GSS Causes Glutathione Synthetase Deficiency (GSS Deficiency) Pathway (1127660)

ncbi summary :

Glutathione S-transferases (GSTs) are a family of enzymes that play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. Based on their biochemical, immunologic, and structural properties, the soluble GSTs are categorized into 4 main classes: alpha, mu, pi, and theta. This GST family member is a polymorphic gene encoding active, functionally different GSTP1 variant proteins that are thought to function in xenobiotic metabolism and play a role in susceptibility to cancer, and other diseases. [provided by RefSeq, Jul 2008]

uniprot summary :

GSTP1: Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Regulates negatively CDK5 activity via p25/p35 translocation to prevent neurodegeneration. Homodimer. Interacts with CDK5. Belongs to the GST superfamily. Pi family. Protein type: Xenobiotic Metabolism - metabolism by cytochrome P450; Other Amino Acids Metabolism - glutathione; Transferase; Xenobiotic Metabolism - drug metabolism - cytochrome P450; EC 2.5.1.18. Chromosomal Location of Human Ortholog: 11q13. Cellular Component: extracellular space; mitochondrion; cytoplasm; plasma membrane; intracellular; nucleus; cytosol; vesicle. Molecular Function: JUN kinase binding; protein binding; glutathione transferase activity; kinase regulator activity. Biological Process: negative regulation of MAP kinase activity; negative regulation of JNK activity; central nervous system development; negative regulation of MAPKKK cascade; negative regulation of acute inflammatory response; negative regulation of interleukin-1 beta production; negative regulation of I-kappaB kinase/NF-kappaB cascade; negative regulation of tumor necrosis factor production; negative regulation of stress-activated MAPK cascade; regulation of stress-activated MAPK cascade; negative regulation of biosynthetic process; response to reactive oxygen species; glutathione metabolic process; negative regulation of fibroblast proliferation; negative regulation of nitric-oxide synthase biosynthetic process; xenobiotic metabolic process; positive regulation of superoxide release; negative regulation of protein kinase activity; negative regulation of apoptosis

size1 :

24-Strip-Wells

price1 :

215 USD

size2 :

48-Strip-Wells

price2 :

410

size3 :

96-Strip-Wells

price3 :

490

size4 :

5x96-Strip-Wells

price4 :

2040

size5 :

10x96-Strip-Wells

price5 :

3590

more info or order :

MyBioSource product webpage