Human NEFL (Neurofilament, Light Polypeptide) ELISA Kit | MyBioSource MBS2510999 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human NEFL (Neurofilament, Light Polypeptide) ELISA Kit

catalog :

MBS2510999

quantity :

24-Strip-Wells

price :

215 USD

product information

catalog number :

MBS2510999

products type :

ELISA Kit

products full name :

Human NEFL (Neurofilament, Light Polypeptide) ELISA Kit

products short name :

[NEFL]

other names :

[neurofilament light polypeptide; Neurofilament light polypeptide; neurofilament light polypeptide; light molecular weight neurofilament protein; neurofilament protein, light chain; neurofilament subunit NF-L; neurofilament triplet L protein; neurofilament, light polypeptide 68kDa; protein phosphatase 1, regulatory subunit 110; neurofilament, light polypeptide; 68 kDa neurofilament protein; Neurofilament triplet L protein]

products gene name :

[NEFL]

other gene names :

[NEFL; NEFL; NFL; NF-L; NF68; CMT1F; CMT2E; PPP1R110; NF68; NFL; NF-L]

uniprot entry name :

NFL_HUMAN

reactivity :

Human

sequence length :

543

specificity :

This kit recognizes Human NE/ELA2 in samples. No significant cross-reactivity or interference between HumanNE/ELA2and analogues was observed.

storage stability :

Store at 4 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 0.78-50ng/mL. Sensitivity: 0.47ng/mL.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, midrange and high level HumanNE/ELA2 were tested 20times on one plate, respectively. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, midrange and high level HumanNE/ELA2 were testedon 3 different plates, 20 replicates in eachplate.

products description :

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human NE/ELA2 concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human NE/ELA2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human NE/ELA2 and Avidin-Horseradish Peroxidase (HRP) conjugate are addedsuccessively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human NE/ELA2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human NE/ELA2. You can calculate the concentration of Human NE/ELA2 in the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

105990539

ncbi acc num :

NP_006149.2

ncbi gb acc num :

NM_006158.4

uniprot acc num :

P07196

ncbi mol weight :

61,517 Da

ncbi pathways :

Activation Of NMDA Receptor Upon Glutamate Binding And Postsynaptic Events Pathway (161033); Amyotrophic Lateral Sclerosis (ALS) Pathway (920975); Amyotrophic Lateral Sclerosis (ALS) Pathway (83099); Amyotrophic Lateral Sclerosis (ALS) Pathway (511); CREB Phosphorylation Through The Activation Of CaMKII Pathway (161040); CREB Phosphorylation Through The Activation Of Ras Pathway (161036); Neuronal System Pathway (106513); Neurotransmitter Receptor Binding And Downstream Transmission In The Postsynaptic Cell Pathway (106534); Post NMDA Receptor Activation Events Pathway (161035); Ras Activation Uopn Ca2+ Infux Through NMDA Receptor Pathway (161037)

ncbi summary :

Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the light chain neurofilament protein. Mutations in this gene cause Charcot-Marie-Tooth disease types 1F (CMT1F) and 2E (CMT2E), disorders of the peripheral nervous system that are characterized by distinct neuropathies. A pseudogene has been identified on chromosome Y. [provided by RefSeq, Oct 2008]

uniprot summary :

NFL: one of the three (L, M, and H) intermediate filament proteins that form neurofilaments. Neurofilaments are involved in the maintenance of neuronal caliber. NF-L is the most abundant of the three neurofilament proteins. Defects cause Charcot-Marie-Tooth disease type 1F (CMT1F). Protein type: Cytoskeletal. Chromosomal Location of Human Ortholog: 8p21. Cellular Component: TSC1-TSC2 complex; growth cone; axon; cytoplasm; neurofilament; cytosol. Molecular Function: protein binding, bridging; protein C-terminus binding; protein domain specific binding; identical protein binding; protein binding; phospholipase binding; structural constituent of cytoskeleton. Biological Process: protein polymerization; response to peptide hormone stimulus; neurofilament bundle assembly; response to toxin; intermediate filament organization; microtubule cytoskeleton organization and biogenesis; axon regeneration in the peripheral nervous system; retrograde axon cargo transport; synaptic transmission; axon transport of mitochondrion; regulation of axon diameter; positive regulation of axonogenesis; negative regulation of neuron apoptosis; locomotion; neuromuscular process controlling balance; anterograde axon cargo transport; neurite morphogenesis; response to corticosterone stimulus. Disease: Charcot-marie-tooth Disease, Axonal, Type 2e; Charcot-marie-tooth Disease, Demyelinating, Type 1f

size1 :

24-Strip-Wells

price1 :

215 USD

size2 :

48-Strip-Wells

price2 :

410

size3 :

96-Strip-Wells

price3 :

490

size4 :

5x96-Strip-Wells

price4 :

2040

size5 :

10x96-Strip-Wells

price5 :

3590