Human WARS (Tryptophanyl tRNA Synthetase) ELISA Kit | MyBioSource MBS2510851 product information

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product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human WARS (Tryptophanyl tRNA Synthetase) ELISA Kit

catalog :

MBS2510851

quantity :

24-Strip-Wells

price :

215 USD

product information

catalog number :

MBS2510851

products type :

ELISA Kit

products full name :

Human WARS (Tryptophanyl tRNA Synthetase) ELISA Kit

products short name :

[WARS]

other names :

[WARS protein; Tryptophan--tRNA ligase, cytoplasmic; tryptophan--tRNA ligase, cytoplasmic; hWRS; interferon-induced protein 53; trpRS; tryptophan tRNA ligase 1, cytoplasmic; tryptophanyl-tRNA synthetase; Interferon-induced protein 53; IFP53; Tryptophanyl-tRNA synthetase; TrpRS; hWRS]

products gene name :

[WARS]

other gene names :

[WARS; WARS; IFI53; IFP53; GAMMA-2; IFI53; WRS; IFP53; TrpRS; hWRS]

uniprot entry name :

SYWC_HUMAN

reactivity :

Human

sequence length :

471

specificity :

This kit recognizes natural and recombinant Human WARS. No significant cross-reactivity or interference between Human WARS and analogues was observed.

storage stability :

Store at 4 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 0.781-50ng/mL. Sensitivity: Min: 0.469ng/mL; Max: 50ng/mL

products description :

Intended Uses This ELISA kit applies to the in vitro quantitative determination of Human WARS concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to WARS. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for WARS and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain WARS, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of WARS. You can calculate the concentration of WARS in the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

63102455

ncbi acc num :

AAH95453.1

uniprot acc num :

P23381

ncbi mol weight :

48,852 Da

ncbi pathways :

Aminoacyl-tRNA Biosynthesis Pathway (83030); Aminoacyl-tRNA Biosynthesis Pathway (424); Aminoacyl-tRNA Biosynthesis, Eukaryotes Pathway (413439); Aminoacyl-tRNA Biosynthesis, Eukaryotes Pathway (468360); Cytosolic TRNA Aminoacylation Pathway (105981); Gene Expression Pathway (105937); Tryptophan Metabolism Pathway (198850); TRNA Aminoacylation Pathway (105980)

ncbi summary :

Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Two forms of tryptophanyl-tRNA synthetase exist, a cytoplasmic form, named WARS, and a mitochondrial form, named WARS2. Tryptophanyl-tRNA synthetase (WARS) catalyzes the aminoacylation of tRNA(trp) with tryptophan and is induced by interferon. Tryptophanyl-tRNA synthetase belongs to the class I tRNA synthetase family. Four transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

uniprot summary :

WARS: Isoform 1, isoform 2 and T1-TrpRS have aminoacylation activity while T2-TrpRS lacks it. Isoform 2, T1-TrpRS and T2-TrpRS possess angiostatic activity whereas isoform 1 lacks it. T2-TrpRS inhibits fluid shear stress-activated responses of endothelial cells. Regulates ERK, Akt, and eNOS activation pathways that are associated with angiogenesis, cytoskeletal reorganization and shear stress-responsive gene expression. Belongs to the class-I aminoacyl-tRNA synthetase family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Amino Acid Metabolism - tryptophan; Translation; Ligase; EC 6.1.1.2. Chromosomal Location of Human Ortholog: 14q32.31. Cellular Component: cytoplasm; nucleus; cytosol. Molecular Function: protein binding; tryptophan-tRNA ligase activity; ATP binding. Biological Process: negative regulation of cell proliferation; tRNA aminoacylation for protein translation; tryptophanyl-tRNA aminoacylation; translation; gene expression; angiogenesis; regulation of angiogenesis

size1 :

24-Strip-Wells

price1 :

215 USD

size2 :

48-Strip-Wells

price2 :

410

size3 :

96-Strip-Wells

price3 :

490

size4 :

5x96-Strip-Wells

price4 :

2040

size5 :

10x96-Strip-Wells

price5 :

3590