catalog number :
MBS2510739
products type :
ELISA Kit
products full name :
Mouse REG4 (Regenerating Islet Derived Protein 4) ELISA Kit
products short name :
[REG4]
other names :
[regenerating islet-derived protein 4 isoform 1; Regenerating islet-derived protein 4; regenerating islet-derived protein 4; REG-4; REG-like protein; gastrointestinal secretory protein; reg IV; regenerating gene type IV; regenerating islet-derived protein IV; regenerating islet-derived family, member 4; Gastrointestinal secretory protein; REG-like protein; Regenerating islet-derived protein IV; Reg IV]
products gene name :
[REG4]
other gene names :
[REG4; REG4; GISP; RELP; REG-IV; GISP; RELP; REG-4; Reg IV]
uniprot entry name :
REG4_HUMAN
specificity :
This kit recognizes natural and recombinant Mouse REG4. No significant cross-reactivity or interference between Mouse REG4 and analogues was observed.
storage stability :
Store at 4 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 46.875-3000pg/mL. Sensitivity: Min: 28.125pg/mL; Max: 3000pg/mL
other info2 :
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Mouse REG4 concentrations in serum, plasma and other biological fluids.
products description :
. Principle of the assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to REG4. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for REG4 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain REG4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of REG4. You can calculate the concentration of REG4 in the samples by comparing the OD of the samples to the standard curve.
ncbi acc num :
NP_114433.1
ncbi gb acc num :
NM_032044.3
ncbi mol weight :
14,993 Da
uniprot summary :
REG4: Calcium-independent lectin displaying mannose-binding specificity and able to maintain carbohydrate recognition activity in an acidic environment. May be involved in inflammatory and metaplastic responses of the gastrointestinal epithelium. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 1p13.1-p12. Cellular Component: cytoplasm; extracellular region. Molecular Function: heparin binding; calcium ion binding
size5 :
10x96-Strip-Wells