catalog number :
MBS2510637
products type :
ELISA Kit
products full name :
Monkey MIP-1beta (Macrophage Inflammatory Protein 1 Beta) ELISA Kit
products short name :
MIP-1beta
other names :
MIP-1beta; C-C motif chemokine 4 homolog; C-C motif chemokine 4 homolog; MIP-1-beta; chemokine-like ligand 1; macrophage inflammatory protein 1-beta homolog; small-inducible cytokine A4 homolog; Macrophage inflammatory protein 1-beta homolog; MIP-1-beta; Small-inducible cytokine A4 homolog
products gene name :
MIP-1beta
other gene names :
CCL4; CCL4; SCYA4; MIP-1beta; SCYA4; MIP-1-beta
uniprot entry name :
CCL4_CHICK
specificity :
This kit recognizes natural and recombinant Monkey MIP-1beta. No significant cross-reactivity or interference between Monkey MIP-1beta and analogues was observed.
storage stability :
Store at 4 degree C.
other info1 :
Samples: Serum, plasma and other biological fluids. Assay Type: Sandwich. Detection Range: 31.25-2000pg/mL. Sensitivity: The minimum detectable dose of Monkey MIP-1beta is 18.75pg/mL (The sensitivity of this assay, or lowest detectable limit (LDL) was defined as the lowest protein concentration that could be differentiated from zero).
other info2 :
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Monkey MIP-1beta concentrations in serum, plasma and other biological fluids.
products description :
Principle of the assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to MIP-1beta. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for MIP-1beta and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain MIP-1beta, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of MIP-1beta. You can calculate the concentration of MIP-1beta in the samples by comparing the OD of the samples to the standard curve.
ncbi acc num :
ABO77445.1
ncbi mol weight :
9,969 Da
ncbi pathways :
Cytokine-cytokine Receptor Interaction Pathway (119307); Cytokine-cytokine Receptor Interaction Pathway (460); Cytosolic DNA-sensing Pathway (125152); Cytosolic DNA-sensing Pathway (124833); Salmonella Infection Pathway (375175); Salmonella Infection Pathway (375149); Toll-like Receptor Signaling Pathway (117308); Toll-like Receptor Signaling Pathway (487)