Human SEMA3A (Semaphorin 3A) ELISA Kit | MyBioSource MBS2510626 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human SEMA3A (Semaphorin 3A) ELISA Kit

catalog :

MBS2510626

quantity :

48-Strip-Wells

price :

410 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2510626

products type :

ELISA Kit

products full name :

Human SEMA3A (Semaphorin 3A) ELISA Kit

products short name :

SEMA3A

other names :

semaphorin-3A; Semaphorin-3A; semaphorin-3A; collapsin 1; semaphorin D; semaphorin III; semaphorin L; sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A; Semaphorin III; Sema III

products gene name :

SEMA3A

other gene names :

SEMA3A; SEMA3A; HH16; SemD; COLL1; SEMA1; SEMAD; SEMAL; coll-1; Hsema-I; SEMAIII; Hsema-III; SEMAD; Sema III

uniprot entry name :

SEM3A_HUMAN

reactivity :

Human

sequence length :

771

specificity :

This kit recognizes natural and recombinant Human SEMA3A. No significant cross-reactivity or interference between Human SEMA3A and analogues was observed.

storage stability :

Store at 4 degree C.

other info1 :

Samples: Serum, plasma and other biological fluids. Assay Type: Sandwich. Detection Range: 0.156-10ng/mL. Sensitivity: 0.094ng/mL

products description :

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human SEMA3A concentrations in serum, plasma and other biological fluids. Principle of the Assay This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to SEMA3A. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for SEMA3A and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain SEMA3A, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of SEMA3A. You can calculate the concentration of SEMA3A in the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

5174673

ncbi acc num :

NP_006071.1

ncbi gb acc num :

NM_006080.2

uniprot acc num :

Q14563

ncbi mol weight :

88,889 Da

ncbi pathways :

Axon Guidance Pathway (83065); Axon Guidance Pathway (476); Axon Guidance Pathway (105688); CRMPs In Sema3A Signaling Pathway (119525); Developmental Biology Pathway (477129); SEMA3A-Plexin Repulsion Signaling By Inhibiting Integrin Adhesion Pathway (119523); Sema3A PAK Dependent Axon Repulsion Pathway (119524); Semaphorin Interactions Pathway (119519)

ncbi summary :

This gene is a member of the semaphorin family and encodes a protein with an Ig-like C2-type (immunoglobulin-like) domain, a PSI domain and a Sema domain. This secreted protein can function as either a chemorepulsive agent, inhibiting axonal outgrowth, or as a chemoattractive agent, stimulating the growth of apical dendrites. In both cases, the protein is vital for normal neuronal pattern development. Increased expression of this protein is associated with schizophrenia and is seen in a variety of human tumor cell lines. Also, aberrant release of this protein is associated with the progression of Alzheimer's disease. [provided by RefSeq, Jul 2008]

uniprot summary :

SEMA3A: Induces the collapse and paralysis of neuronal growth cones. Could serve as a ligand that guides specific growth cones by a motility-inhibiting mechanism. Binds to the complex neuropilin-1/plexin-1. Belongs to the semaphorin family. Protein type: Secreted; Secreted, signal peptide; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 7p12.1. Cellular Component: membrane; axon; dendrite; extracellular region. Molecular Function: chemorepellent activity; receptor activity; semaphorin receptor binding. Biological Process: trigeminal nerve structural organization; facial nerve structural organization; axon guidance; regulation of heart rate; apoptosis; dendrite morphogenesis; olfactory bulb development; neuron migration; axonal fasciculation; negative chemotaxis; negative regulation of axon extension involved in axon guidance; axon extension involved in axon guidance; gonadotrophin-releasing hormone neuronal migration to the hypothalamus; sympathetic nervous system development; branchiomotor neuron axon guidance; regulation of axon extension involved in axon guidance; nerve development; sensory system development. Disease: Hypogonadotropic Hypogonadism 16 With Or Without Anosmia

size1 :

48-Strip-Wells

price1 :

410 USD

size2 :

96-Strip-Wells

price2 :

490

more info or order :

MyBioSource product webpage