catalog number :
MBS2510536
products type :
ELISA Kit
products full name :
Rat PTHRP (Parathyroid Hormone Related Protein) ELISA Kit
products short name :
[PTHRP]
other names :
[parathyroid hormone-related protein isoform 1 preproprotein; Parathyroid hormone-related protein; parathyroid hormone-related protein; PTH-rP; PTH-related protein; osteostatin; parathyroid hormone-like hormone preproprotein; parathyroid hormone-like related protein; parathyroid hormone-like hormone; Parathyroid hormone-like protein; PLP]
products gene name :
[PTHrP]
other gene names :
[PTHLH; PTHLH; HHM; PLP; BDE2; PTHR; PTHRP; PTHRP; PTH-rP; PTHrP; PLP]
uniprot entry name :
PTHR_HUMAN
specificity :
This kit recognizes Rat PTH in samples. No Significant cross-reactivity or interference between Rat PTH and analogues was observed.
storage stability :
Store at 4 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 12.5-800 pg/mL. Sensitivity: 7.5 pg/mL
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat PTH were tested 20 times on one plate, respectively. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat PTH were tested on 3 different plates, 20 replicates in each plate.
products description :
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Rat PTH concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat PTH. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat PTH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat PTH, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Rat PTH. You can calculate the concentration of Rat PTH in the samples by comparing the OD of the samples to the standard curve.
ncbi acc num :
NP_945317.1
ncbi gb acc num :
NM_198966.1
ncbi mol weight :
23,942 Da
ncbi pathways :
Class B/2 (Secretin Family Receptors) Pathway (106378); Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway (1127664); Disease Pathway (530764); Endochondral Ossification Pathway (198812); G Alpha (s) Signalling Events Pathway (119549); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020); Metabolic Disorders Of Biological Oxidation Enzymes Pathway (1127637); Signal Transduction Pathway (477114); Signaling By GPCR Pathway (106356)
size5 :
10x96-Strip-Wells