catalog number :
MBS2510454
products type :
ELISA Kit
products full name :
Mouse TIMP-1 (Tissue Inhibitors of Metalloproteinase 1) ELISA Kit
products short name :
[TIMP-1]
other names :
[TIMP-1; Metalloproteinase inhibitor 1; metalloproteinase inhibitor 1; EG-1; TIMP-1; embryogenin-1; tissue inhibitor of metalloproteinase 1 (erythroid potentiating activity, collagenase inhibitor); tissue inhibitor of metalloproteinases 1; TIMP metallopeptidase inhibitor 1<; Embryogenin-1; EG-1; Tissue inhibitor of metalloproteinases 1; TIMP-1]
products gene name :
[TIMP-1]
other gene names :
[TIMP1; TIMP1; EG-1; TIMP-1]
uniprot entry name :
TIMP1_BOVIN
specificity :
This kit recognizes Mouse TGF-alpha in samples. No significant cross-reactivity or interference between Mouse TGF-alpha and analogues was observed.
storage stability :
Store at 4 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 15.63-1000pg/mL. Sensitivity: 9.38pg/mL.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse TGF-alpha were tested 20 times on one plate, respectively. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse TGF-alpha were tested on 3 different plates, 20 replicates in each plate.
products description :
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Mouse TGF-alpha concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse TGF-alpha. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse TGF-alpha and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse TGF-alpha, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse TGF-alpha. You can calculate the concentration of Mouse TGF-alpha in the samples by comparing the OD of the samples to the standard curve.
ncbi acc num :
AAB30892.1
ncbi mol weight :
23,032 Da
ncbi pathways :
Activation Of Matrix Metalloproteinases Pathway (1120055); Degradation Of The Extracellular Matrix Pathway (1120054); Extracellular Matrix Organization Pathway (1120041); HIF-1 Signaling Pathway (695207); Hemostasis Pathway (1120392); Matrix Metalloproteinases Pathway (198565); Platelet Activation, Signaling And Aggregation Pathway (1120402); Platelet Degranulation Pathway (1120414); Response To Elevated Platelet Cytosolic Ca2+ Pathway (1120412)
uniprot summary :
Metalloproteinase inhibitor that functions by forming one to one complexes with target metalloproteinases, such as collagenases, and irreversibly inactivates them by binding to their catalytic zinc cofactor. Acts on MMP1, MMP2, MMP3, MMP7, MMP8, MMP9, MMP10, MMP11, MMP12, MMP13 and MMP16. Does not act on MMP14. Also functions as a growth factor that regulates cell differentiation, migration and cell death and activates cellular signaling cascades via CD63 and ITGB1. Plays a role in integrin signaling.
size5 :
10x96-Strip-Wells