ELISA/assay

Rabbit FOXP3 (Forkhead Box Protein P3) ELISA Kit

MBS2510451

48-Strip-Wells

450 USD

ELISA Kit

Rabbit FOXP3 (Forkhead Box Protein P3) ELISA Kit

FOXP3

forkhead box protein P3 isoform a; Forkhead box protein P3; forkhead box protein P3; FOXP3delta7; immune dysregulation, polyendocrinopathy, enteropathy, X-linked; immunodeficiency, polyendocrinopathy, enteropathy, X-linked; scurfin; forkhead box P3; Scurfin

FOXP3

FOXP3; FOXP3; JM2; AIID; IPEX; PIDX; XPID; DIETER; IPEX

FOXP3_HUMAN

Rabbit

431

This assay has high sensitivity and excellent specificity for detection of Rabbit FOXP3. No significant cross-reactivity or interference between Rabbit FOXP3 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Rabbit FOXP3 and all the analogues, therefore, cross reaction may still exist.

Store at 4 degree C.

Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich

Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of FOXP3 in Rabbit serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rabbit FOXP3. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Rabbit FOXP3 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rabbit FOXP3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Rabbit FOXP3. You can calculate the concentration of Rabbit FOXP3 in the samples by comparing the OD of the samples to the standard curve.

31982943

NP_054728.2

NM_014009.3

Q9BZS1

44,407 Da

Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway (137993); IL2 Signaling Events Mediated By STAT5 Pathway (137988); Inflammatory Bowel Disease (IBD) Pathway (842771); Inflammatory Bowel Disease (IBD) Pathway (842797)

The protein encoded by this gene is a member of the forkhead/winged-helix family of transcriptional regulators. Defects in this gene are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX), also known as X-linked autoimmunity-immunodeficiency syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

FOXP3: Probable transcription factor. Plays a critical role in the control of immune response. Interacts with IKZF3. 3 isoforms of the human protein are produced by alternative splicing. Protein type: C2H2-type zinc finger protein; DNA-binding; Cell cycle regulation; Transcription factor. Chromosomal Location of Human Ortholog: Xp11.23. Cellular Component: protein complex; cytoplasm; nucleus. Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; histone acetyltransferase binding; NF-kappaB binding; protein binding; protein homodimerization activity; NFAT protein binding; sequence-specific DNA binding; metal ion binding; histone deacetylase binding; transcription factor activity; transcription corepressor activity. Biological Process: negative regulation of interleukin-10 production; positive regulation of transcription, DNA-dependent; negative regulation of cytokine secretion; myeloid cell homeostasis; T cell receptor signaling pathway; negative regulation of interleukin-2 production; negative regulation of interleukin-6 production; T cell homeostasis; tolerance induction to self antigen; negative regulation of transcription factor activity; response to virus; negative regulation of immune response; cytokine production; negative regulation of T cell cytokine production; positive regulation of peripheral T cell tolerance induction; inhibition of NF-kappaB transcription factor; regulation of T cell anergy; negative regulation of interleukin-5 production; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; inhibition of CREB transcription factor; negative regulation of cytokine biosynthetic process; transcription from RNA polymerase II promoter; negative regulation of interleukin-4 production; T cell activation; negative regulation of activated T cell proliferation; negative regulation of transcription from RNA polymerase II promoter; negative regulation of histone acetylation; negative regulation of interferon-gamma biosynthetic process; positive regulation of interleukin-4 production; negative regulation of cell proliferation; CD4-positive, CD25-positive, alpha-beta regulatory T cell lineage commitment; negative regulation of isotype switching to IgE isotypes; negative regulation of T cell proliferation; B cell homeostasis; regulation of transcription, DNA-dependent; negative regulation of interferon-gamma production; positive regulation of T cell anergy; regulation of isotype switching to IgG isotypes; positive regulation of transforming growth factor-beta1 production; negative regulation of chronic inflammatory response; negative regulation of histone deacetylation; T cell mediated immunity; positive regulation of immature T cell proliferation in the thymus; negative regulation of tumor necrosis factor production; chromatin remodeling; negative regulation of interleukin-17 production; positive regulation of CD4-positive, CD25-positive, alpha-beta regulatory T cell differentiation; negative regulation of interleukin-2 biosynthetic process; positive regulation of histone acetylation. Disease: Diabetes Mellitus, Insulin-dependent; Immunodysregulation, Polyendocrinopathy, And Enteropathy, X-linked

48-Strip-Wells

450 USD

96-Strip-Wells

535