Rat CA2 (Carbonic Anhydrase II) ELISA Kit | MyBioSource MBS2510418 product information

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product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rat CA2 (Carbonic Anhydrase II) ELISA Kit

catalog :

MBS2510418

quantity :

24-Strip-Wells

price :

215 USD

product information

catalog number :

MBS2510418

products type :

ELISA Kit

products full name :

Rat CA2 (Carbonic Anhydrase II) ELISA Kit

products short name :

[CA2]

other names :

[CA2, partial; Carbonic anhydrase 2; carbonic anhydrase 2; carbonate dehydratase II; carbonic anhydrase B; carbonic anhydrase C; carbonic dehydratase; epididymis luminal protein 76; carbonic anhydrase II; Carbonate dehydratase II; Carbonic anhydrase C; CAC; Carbonic anhydrase II; CA-II]

products gene name :

[CA2]

other gene names :

[CA2; CA2; CAC; CAII; Car2; CA-II; HEL-76; CAC; CA-II]

uniprot entry name :

CAH2_HUMAN

reactivity :

Rat

sequence length :

260

specificity :

This kit recognizes natural and recombinant Rat CA2. No significant cross-reactivity or interference between Rat CA2 and analogues was observed.

storage stability :

Store at 4 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 2.813-180ng/mL. Sensitivity: Min: 1.688ng/mL; Max: 180ng/mL

products description :

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Rat CA2 concentrations in serum, plasma and other biological fluids. Principle of the Assay This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to CA2. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for CA2 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain CA2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of CA2. You can calculate the concentration of CA2 in the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

49456705

ncbi acc num :

CAG46673.1

uniprot acc num :

P00918

ncbi mol weight :

29,246 Da

ncbi pathways :

Bile Secretion Pathway (193146); Bile Secretion Pathway (193095); Collecting Duct Acid Secretion Pathway (147586); Collecting Duct Acid Secretion Pathway (147560); Erythrocytes Take Up Carbon Dioxide And Release Oxygen Pathway (645347); Erythrocytes Take Up Oxygen And Release Carbon Dioxide Pathway (645348); Gastric Acid Secretion Pathway (154409); Gastric Acid Secretion Pathway (154383); Metabolism Pathway (477135); Nitrogen Metabolism Pathway (83025)

ncbi summary :

The protein encoded by this gene is one of several isozymes of carbonic anhydrase, which catalyzes reversible hydration of carbon dioxide. Defects in this enzyme are associated with osteopetrosis and renal tubular acidosis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2014]

uniprot summary :

CA2: Essential for bone resorption and osteoclast differentiation. Reversible hydration of carbon dioxide. Can hydrates cyanamide to urea. Involved in the regulation of fluid secretion into the anterior chamber of the eye. Interacts with SLC4A4. Interaction with SLC4A7 regulates SLC4A7 transporter activity. Activated by X-ray, histamine, L-adrenaline, L- and D-phenylalanine, L- and D-histidine, L-His-OMe and beta-Ala- His (carnosine). Competitively inhibited by saccharin, thioxolone, coumarins, 667-coumate, celecoxib (Celebrex), valdecoxib (Bextra), SC-125, SC-560, diclofenac, acetate, azide, bromide, sulfonamide derivatives such as acetazolamide (AZA), methazolamide (MZA), ethoxzolamide (EZA), dichlorophenamide (DCP), brinzolamide, dansylamide, thiabendazole-5-sulfonamide, trifluoromethane sulfonamide and N-hydroxysulfamide, fructose-based sugar sulfamate RWJ-37497, and Foscarnet (phosphonoformate trisodium salt). Repressed strongly by hydrogen sulfide(HS) and weakly by nitrate (NO(3)). Esterase activity weakly reduced by cyanamide. N- hydroxyurea interfers with zinc binding and inhibit activity. Belongs to the alpha-carbonic anhydrase family. Protein type: Lyase; Energy Metabolism - nitrogen; EC 4.2.1.1. Chromosomal Location of Human Ortholog: 8q22. Cellular Component: extracellular space; microvillus; axon; basolateral plasma membrane; apical part of cell; cytoplasm; plasma membrane; cytosol; myelin sheath. Molecular Function: protein binding; carbonate dehydratase activity; zinc ion binding. Biological Process: secretion; positive regulation of osteoclast differentiation; positive regulation of cellular pH reduction; positive regulation of synaptic transmission, GABAergic; one-carbon compound metabolic process; odontogenesis of dentine-containing teeth; response to estrogen stimulus; bicarbonate transport; response to zinc ion; regulation of intracellular pH; positive regulation of bone resorption; morphogenesis of an epithelium; kidney development; response to pH. Disease: Osteopetrosis, Autosomal Recessive 3

size1 :

24-Strip-Wells

price1 :

215 USD

size2 :

48-Strip-Wells

price2 :

410

size3 :

96-Strip-Wells

price3 :

490

size4 :

5x96-Strip-Wells

price4 :

2040

size5 :

10x96-Strip-Wells

price5 :

3590