catalog number :
MBS2510385
products type :
ELISA Kit
products full name :
Human IL-1beta (Interleukin 1 Beta) ELISA Kit
products short name :
IL-1beta
other names :
interleukin 1, beta; interleukin 1, beta; interleukin-1 beta
products gene name :
IL-1beta
other gene names :
IL1B2; IL1B; IL-1beta
specificity :
This kit recognizes natural and recombinant Human IL-1beta. No significant cross-reactivity or interference between Human IL-1beta and analogues was observed.
storage stability :
Store at 4 degree C.
other info1 :
Samples: Serum, plasma, saliva and other biological fluids. Assay Type: Sandwich. Detection Range: 7.813-500pg/mL. Sensitivity: The minimum detectable dose of Human IL-1beta is 4.688pg/mL
products description :
Intended Uses This ELISA kit applies to the in vitro quantitative determination of Human IL-1beta concentrations in serum, plasma, saliva and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to IL-1beta. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for IL-1beta and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain IL-1beta, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of IL-1beta. You can calculate the concentration of IL-1beta in the samples by comparing the OD of the samples to the standard curve.
ncbi acc num :
NP_001005149.1
ncbi gb acc num :
NM_001005149.1
ncbi pathways :
African Trypanosomiasis Pathway (194379); African Trypanosomiasis Pathway (194323); Alzheimer's Disease Pathway (84508); Alzheimer's Disease Pathway (509); Amoebiasis Pathway (167313); Amoebiasis Pathway (167191); Apoptosis Pathway (84472); Apoptosis Pathway (470); Chagas Disease (American Trypanosomiasis) Pathway (147807); Chagas Disease (American Trypanosomiasis) Pathway (147795)