Human TNNI1 (Troponin I Type 1, Slow Skeletal) ELISA Kit | MyBioSource MBS2510383 product information

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product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human TNNI1 (Troponin I Type 1, Slow Skeletal) ELISA Kit

catalog :

MBS2510383

quantity :

24-Strip-Wells

price :

215 USD

product information

catalog number :

MBS2510383

products type :

ELISA Kit

products full name :

Human TNNI1 (Troponin I Type 1, Slow Skeletal) ELISA Kit

products short name :

[TNNI1]

other names :

[troponin I, slow skeletal muscle; Troponin I, slow skeletal muscle; troponin I, slow skeletal muscle; troponin I, slow-twitch isoform; troponin I type 1 (skeletal, slow); Troponin I, slow-twitch isoform]

products gene name :

[TNNI1]

other gene names :

[TNNI1; TNNI1; TNN1; SSTNI]

uniprot entry name :

TNNI1_HUMAN

reactivity :

Human

sequence length :

187

specificity :

This kit recognizes Human TNF-alpha in samples. No Significant cross-reactivity or interference between Human TNF-alpha and analogues was observed.

storage stability :

Store at 4 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 7.81-500 pg/mL. Sensitivity: 4.69 pg/mL

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human TNF-alpha were tested 20 times on one plate, respectively. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human TNF-alpha were tested on 3 different plates, 20 replicates in each plate.

products description :

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human TNF-alpha concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human TNF-alpha. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TNF-alpha and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TNF-alpha, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human TNF-alpha. You can calculate the concentration of Human TNF-alpha in the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

56682969

ncbi acc num :

NP_003272.3

ncbi gb acc num :

NM_003281.3

uniprot acc num :

P19237

ncbi mol weight :

21,692 Da

ncbi pathways :

Muscle Contraction Pathway (106261); Striated Muscle Contraction Pathway (198903); Striated Muscle Contraction Pathway (106262)

ncbi summary :

Troponin proteins associate with tropomyosin and regulate the calcium sensitivity of the myofibril contractile apparatus of striated muscles. Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. The TnI-fast and TnI-slow genes are expressed in fast-twitch and slow-twitch skeletal muscle fibers, respectively, while the TnI-cardiac gene is expressed exclusively in cardiac muscle tissue. This gene encodes the Troponin-I-skeletal-slow-twitch protein. This gene is expressed in cardiac and skeletal muscle during early development but is restricted to slow-twitch skeletal muscle fibers in adults. The encoded protein prevents muscle contraction by inhibiting calcium-mediated conformational changes in actin-myosin complexes. [provided by RefSeq, Jul 2008]

uniprot summary :

TNNI1: Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. Belongs to the troponin I family. Protein type: Cytoskeletal; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 1q31.3. Cellular Component: troponin complex; cytosol. Molecular Function: metal ion binding; actin binding. Biological Process: regulation of striated muscle contraction; ventricular cardiac muscle morphogenesis; muscle filament sliding

size1 :

24-Strip-Wells

price1 :

215 USD

size2 :

48-Strip-Wells

price2 :

410

size3 :

96-Strip-Wells

price3 :

490

size4 :

5x96-Strip-Wells

price4 :

2040

size5 :

10x96-Strip-Wells

price5 :

3590