catalog number :
MBS2510380
products type :
ELISA Kit
products full name :
Rat ALD (Aldosterone) ELISA Kit
products short name :
[ALD]
other names :
[Ald; Alanine dehydrogenase]
products gene name :
[ALD]
uniprot entry name :
A7Z8A3_BACA2
specificity :
This kit recognizes natural and recombinant Rat ALD. No significant cross-reactivity or interference between Rat ALD and analogues was observed.
storage stability :
Store at 4 degree C.
other info1 :
Samples: Serum, Plasma, Biological Fluids. Assay Type: Competitive. Detection Range: 31.25-2000pg/mL. Sensitivity: Min: 18.75pg/mL; Max: 2000pg/mL
products description :
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Rat ALD concentrations in serum, plasma and other biological fluids. Principle of the Assay This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with ALD. During the reaction, ALD in the sample or standard competes with a fixed amount of ALD on the solid phase supporter for sites on the Biotinylated Detection Ab specific to ALD. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of ALD in the samples is then determined by comparing the OD of the samples to the standard curve.
ncbi acc num :
ABS75229.1
ncbi mol weight :
39,315 Da
size5 :
10x96-Strip-Wells