catalog number :
MBS2510368
products type :
ELISA Kit
products full name :
Human C1r (Complement Component 1R) ELISA Kit
products short name :
[C1r]
other names :
[complement C1r subcomponent; Complement C1r subcomponent; complement C1r subcomponent; complement component 1, r subcomponent; Complement component 1 subcomponent rCleaved into the following 2 chains:Complement C1r subcomponent heavy chain; Complement C1r subcomponent light chain]
products gene name :
[Clr]
other gene names :
[C1R; C1R]
uniprot entry name :
C1R_HUMAN
specificity :
This kit recognizes natural and recombinantHumanClr. No significant cross-reactivity or interference between HumanClr and analogues was observed.
storage stability :
Store at 4 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 0.313-20ng/mL. Sensitivity: Min: 0.188ng/mL; Max: 20ng/mL
products description :
Intended Uses: This ELISA kit applies to the invitro quantitative determination of HumanClrconcentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Clr. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Clrand Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Clr, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Clr.You can calculate the concentration of Clrin the samples by comparing the OD of the samples to the standard curve.
ncbi acc num :
NP_001724.3
ncbi gb acc num :
NM_001733.4
ncbi mol weight :
80,119 Da
ncbi pathways :
Classical Antibody-mediated Complement Activation Pathway (106409); Complement Activation, Classical Pathway (198823); Complement And Coagulation Cascades Pathway (198880); Complement And Coagulation Cascades Pathway (83073); Complement And Coagulation Cascades Pathway (484); Complement Cascade Pathway (106405); Creation Of C4 And C2 Activators Pathway (106407); Immune System Pathway (106386); Initial Triggering Of Complement Pathway (106406); Innate Immune System Pathway (106387)
uniprot summary :
C1R: C1r B chain is a serine protease that combines with C1q and C1s to form C1, the first component of the classical pathway of the complement system. Belongs to the peptidase S1 family. Protein type: EC 3.4.21.41; Protease. Chromosomal Location of Human Ortholog: 12p13. Cellular Component: extracellular region. Molecular Function: protein binding; serine-type peptidase activity; serine-type endopeptidase activity; calcium ion binding. Biological Process: innate immune response; immune response; proteolysis; complement activation, classical pathway; complement activation. Disease: Complement Component C1r/c1s Deficiency
size5 :
10x96-Strip-Wells