catalog number :
MBS2510341
products type :
ELISA Kit
products full name :
Human VEGF-A (Vascular Endothelial Cell Growth Factor A) ELISA Kit
products short name :
VEGF-A
other names :
VEGF; VEGF; VEGF
products gene name :
VEGF-A
uniprot entry name :
Q2F842_ORFV
specificity :
This kit recognizes natural and recombinant Human VEGF-A. No significant cross-reactivity or interference between Human VEGF-A and analogues was observed.
storage stability :
Store at 4 degree C.
other info1 :
Samples: Serum, plasma and other biological fluids. Assay Type: Sandwich. Detection Range: 31.25-2000pg/mL. Sensitivity: The minimum detectable dose of Human VEGF-A is 18.75pg/mL (The sensitivity of this assay, or lowest detectable limit (LDL) was defined as the lowest protein concentration that could be differentiated from zero).
other info2 :
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human VEGF-A concentrations in serum, plasma and other biological fluids.
products description :
Principle of the assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to VEGF-A. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for VEGF-A and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain VEGF-A, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of VEGF-A. You can calculate the concentration of VEGF-A in the samples by comparing the OD of the samples to the standard curve.
ncbi acc num :
AHD25648.1
ncbi mol weight :
14,729 Da