catalog number :
MBS2510320
products type :
ELISA Kit
products full name :
Mouse fT3 (Free Triiodothyronine) ELISA Kit
products short name :
[fT3]
other names :
[FT3; FT2 protein; FT2 protein]
products gene name :
[fT3]
uniprot entry name :
U5U6H8_ALLCE
specificity :
This kit recognizes Human sST2 in samples. No s ignificant cross-reactivity or interference between Human sST2 and analogues was observed.
storage stability :
Store at 4 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 31.25-2000 pg/mL. Sensitivity: 18.75pg/mL.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, midrange and high level Human sST2 were tested 20times on one plate, respectively. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, midrange and high level Human sST2 were testedon 3 different plates, 20 replicates in each plate.
products description :
Intended Uses: This ELISA kit can be applied to the in vitro quantitative determination of Human sST2 concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been precoated with an antibody specific to Human sST2. Standards or samples are added to appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibodies specific for Human sST2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. After incubation, free components are washed away. Then the Substrate Reagent is added to each well, only those wells that contain Human sST2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction will be terminated by adding Stop Solution and appears yellow in color. The optical density (OD) can be measured with spectrophotometry at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human sST2. The concentration of Human sST2 in samples can be calculated by comparing the OD of the samples with the standard curve.
ncbi acc num :
AGZ20209.1
ncbi mol weight :
19,844 Da
size5 :
10x96-Strip-Wells