catalog number :
MBS2510262
products type :
ELISA Kit
products full name :
Mouse ECE1 (Endothelin Converting Enzyme 1) ELISA Kit
products short name :
[ECE1]
other names :
[endothelin-converting enzyme 1; Endothelin-converting enzyme 1; endothelin-converting enzyme 1; ECE-1; endothelin converting enzyme 1]
products gene name :
[ECE1]
other gene names :
[Ece1; Ece1; Ece; ECE-1]
uniprot entry name :
ECE1_RAT
specificity :
This kit recognizes MouseDPD in samples. No significantcross-reactivity or interference between MouseDPD and analogues was observed.
storage stability :
Store at 4 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Competitive. Detection Range: 3.13-200ng/mL. Sensitivity: 1.88ng/mL.
products description :
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Mouse DPD concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Mouse DPD. During the reaction, Mouse DPD in the sample or standard competes with a fixed amount of Mouse DPD on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Mouse DPD. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Mouse DPD in the samples is then determined by comparing the OD of the samples to the standard curve.
ncbi acc num :
NP_446048.2
ncbi gb acc num :
NM_053596.2
ncbi mol weight :
87,011 Da
ncbi summary :
catalyzes the conversion of the big endothelin precursor (big ET-1) to the vasoconstrictive peptide endothelin [RGD, Feb 2006]
uniprot summary :
Converts big endothelin-1 to endothelin-1.
size5 :
10x96-Strip-Wells