Mouse TPO (Thrombopoietin) ELISA Kit | MyBioSource MBS2510249 product information

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product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse TPO (Thrombopoietin) ELISA Kit

catalog :

MBS2510249

quantity :

24-Strip-Wells

price :

190 USD

product information

catalog number :

MBS2510249

products type :

ELISA Kit

products full name :

Mouse TPO (Thrombopoietin) ELISA Kit

products short name :

[TPO]

other names :

[thyroid peroxidase isoform d; Thyroid peroxidase; thyroid peroxidase; thyroid microsomal antigen; thyroperoxidase; thyroid peroxidase]

products gene name :

[TPO]

other gene names :

[TPO; TPO; MSA; TPX; TDH2A; TPO]

uniprot entry name :

PERT_HUMAN

reactivity :

Mouse

sequence length :

889

specificity :

This kit recognizes natural and recombinant Mouse TPO. No significant cross-reactivity or interference between Mouse TPO and analogues was observed.

storage stability :

Store at 4 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 7.813-500pg/mL. Sensitivity: Min: 4.688pg/mL; Max: 500pg/mL

other info2 :

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Mouse TPO concentrations in serum, plasma and other biological fluids.

products description :

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to TPO. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for TPO and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain TPO, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of TPO. You can calculate the concentration of TPO in the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

28558988

ncbi acc num :

NP_783652.1

ncbi gb acc num :

NM_175721.3

uniprot acc num :

P07202

ncbi mol weight :

92,063 Da

ncbi pathways :

Amine-derived Hormones Pathway (160983); Autoimmune Thyroid Disease Pathway (83121); Autoimmune Thyroid Disease Pathway (533); Metabolic Pathways (132956); Metabolism Pathway (477135); Metabolism Of Amino Acids And Derivatives Pathway (106169); Thyroid Hormone Biosynthesis, Tyrosine = Triiodothyronine/thyroxine Pathway (413358); Thyroid Hormone Biosynthesis, Tyrosine = Triiodothyronine/thyroxine Pathway (468236); Thyroid Hormone Synthesis Pathway (835410); Thyroid Hormone Synthesis Pathway (839541)

ncbi summary :

This gene encodes a membrane-bound glycoprotein. The encoded protein acts as an enzyme and plays a central role in thyroid gland function. The protein functions in the iodination of tyrosine residues in thyroglobulin and phenoxy-ester formation between pairs of iodinated tyrosines to generate the thyroid hormones, thyroxine and triiodothyronine. Mutations in this gene are associated with several disorders of thyroid hormonogenesis, including congenital hypothyroidism, congenital goiter, and thyroid hormone organification defect IIA. Multiple transcript variants encoding distinct isoforms have been identified for this gene, but the full-length nature of some variants has not been determined. [provided by RefSeq, May 2011]

uniprot summary :

TPO: Iodination and coupling of the hormonogenic tyrosines in thyroglobulin to yield the thyroid hormones T(3) and T(4). An alternative splicing in the thyroperoxidase mRNA can cause Graves disease. Defects in TPO are the cause of thyroid dyshormonogenesis 2A (TDH2A). A disorder due to defective conversion of accumulated iodide to organically bound iodine. The iodide organification defect can be partial or complete. Belongs to the peroxidase family. XPO subfamily. 8 isoforms of the human protein are produced by alternative splicing. Protein type: Mitochondrial; Oxidoreductase; Membrane protein, integral; EC 1.11.1.8; Amino Acid Metabolism - tyrosine. Chromosomal Location of Human Ortholog: 2p25. Cellular Component: extracellular space; cell surface; mitochondrion; integral to plasma membrane; plasma membrane. Molecular Function: peroxidase activity; heme binding; iodide peroxidase activity; calcium ion binding. Biological Process: hydrogen peroxide catabolic process; embryonic hemopoiesis; thyroid hormone generation; response to oxidative stress; hormone biosynthetic process. Disease: Thyroid Dyshormonogenesis 2a

size1 :

24-Strip-Wells

price1 :

190 USD

size2 :

48-Strip-Wells

price2 :

310

size3 :

96-Strip-Wells

price3 :

355

size4 :

5x96-Strip-Wells

price4 :

1435

size5 :

10x96-Strip-Wells

price5 :

2805