Rat REN (Renin) ELISA Kit | MyBioSource MBS2510108 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rat REN (Renin) ELISA Kit

catalog :

MBS2510108

quantity :

24-Strip-Wells

price :

190 USD

product information

catalog number :

MBS2510108

products type :

ELISA Kit

products full name :

Rat REN (Renin) ELISA Kit

products short name :

[REN]

other names :

[REN, partial; Renin; renin; angiotensin-forming enzyme; angiotensinogenase; renin precursor, renal; renin; Angiotensinogenase]

products gene name :

[REN]

other gene names :

[REN; REN; HNFJ2]

uniprot entry name :

RENI_HUMAN

reactivity :

Rat

sequence length :

specificity :

This kit recognizes natural and recombinantRatREN. No significant cross-reactivity or interference between RatREN and analogues was observed.

storage stability :

Store at 4 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 15.625-1000pg/mL. Sensitivity: Min: 9.375pg/mL; Max: 1000pg/mL

products description :

Intended Uses: This ELISA kit applies to the invitro quantitative determination of RatRENconcentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to REN. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for RENand Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain REN, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of REN.You can calculate the concentration of RENin the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

190996

ncbi acc num :

AAA60262.1

uniprot acc num :

P00797

ncbi mol weight :

44,726 Da

ncbi pathways :

ACE Inhibitor Pathway (198763); Metabolism Of Angiotensinogen To Angiotensins Pathway (685555); Metabolism Of Proteins Pathway (106230); Peptide Hormone Metabolism Pathway (771603); Renin-angiotensin System Pathway (83075); Renin-angiotensin System Pathway (486)

ncbi summary :

Renin catalyzes the first step in the activation pathway of angiotensinogen--a cascade that can result in aldosterone release,vasoconstriction, and increase in blood pressure. Renin, an aspartyl protease, cleaves angiotensinogen to form angiotensin I, which is converted to angiotensin II by angiotensin I converting enzyme, an important regulator of blood pressure and electrolyte balance. Transcript variants that encode different protein isoforms and that arise from alternative splicing and the use of alternative promoters have been described, but their full-length nature has not been determined. Mutations in this gene have been shown to cause familial hyperproreninemia. [provided by RefSeq, Jul 2008]

uniprot summary :

REN: Renin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney. Interacts with ATP6AP2. Interaction with ATP6AP2 results in a 5-fold increased efficiency in angiotensinogen processing. Belongs to the peptidase A1 family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Protease; Secreted, signal peptide; Secreted; EC 3.4.23.15. Chromosomal Location of Human Ortholog: 1q32. Cellular Component: extracellular space; cytoplasm; extracellular region; plasma membrane. Molecular Function: peptidase activity; insulin-like growth factor receptor binding; aspartic-type endopeptidase activity; receptor binding. Biological Process: response to cAMP; hormone-mediated signaling; cell maturation; male gonad development; renin-angiotensin regulation of aldosterone production; drinking behavior; response to lipopolysaccharide; proteolysis; angiotensin maturation; cellular protein metabolic process; regulation of MAPKKK cascade; regulation of blood pressure; beta-amyloid metabolic process; mesonephros development; kidney development. Disease: Renal Tubular Dysgenesis; Hyperuricemic Nephropathy, Familial Juvenile, 2

size1 :

24-Strip-Wells

price1 :

190 USD

size2 :

48-Strip-Wells

price2 :

310

size3 :

96-Strip-Wells

price3 :

355

size4 :

5x96-Strip-Wells

price4 :

1435

size5 :

10x96-Strip-Wells

price5 :

2805