Mouse FOXP3 (Forkhead Box Protein P3) ELISA Kit | MyBioSource MBS2510062 product information

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product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse FOXP3 (Forkhead Box Protein P3) ELISA Kit

catalog :

MBS2510062

quantity :

24-Strip-Wells

price :

215 USD

product information

catalog number :

MBS2510062

products type :

ELISA Kit

products full name :

Mouse FOXP3 (Forkhead Box Protein P3) ELISA Kit

products short name :

[FOXP3]

other names :

[forkhead box protein P3 isoform a; Forkhead box protein P3; forkhead box protein P3; FOXP3delta7; immune dysregulation, polyendocrinopathy, enteropathy, X-linked; immunodeficiency, polyendocrinopathy, enteropathy, X-linked; scurfin; forkhead box P3; Scurfin]

products gene name :

[FXOP3]

other gene names :

[FOXP3; FOXP3; JM2; AIID; IPEX; PIDX; XPID; DIETER; IPEX]

uniprot entry name :

FOXP3_HUMAN

reactivity :

Mouse

sequence length :

431

specificity :

This kit recognizes Mouse FOXO1 in samples. No Significant cross-reactivity or interference between Mouse FOXO1 and analogues was observed.

storage stability :

Store at 4 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 0.16-10 ng/mL. Sensitivity: 0.10 ng/mL

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse FOXO1 were tested 20 times on one plate, respectively. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse FOXO1 were tested on 3 different plates, 20 replicates in each plate.

products description :

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Mouse FOXO1 concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse FOXO1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse FOXO1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse FOXO1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse FOXO1. You can calculate the concentration of Mouse FOXO1 in the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

31982943

ncbi acc num :

NP_054728.2

ncbi gb acc num :

NM_014009.3

uniprot acc num :

Q9BZS1

ncbi mol weight :

44,407 Da

ncbi pathways :

Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway (137993); IL2 Signaling Events Mediated By STAT5 Pathway (137988); Inflammatory Bowel Disease (IBD) Pathway (842771); Inflammatory Bowel Disease (IBD) Pathway (842797)

ncbi summary :

The protein encoded by this gene is a member of the forkhead/winged-helix family of transcriptional regulators. Defects in this gene are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX), also known as X-linked autoimmunity-immunodeficiency syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

uniprot summary :

FOXP3: Probable transcription factor. Plays a critical role in the control of immune response. Interacts with IKZF3. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Cell cycle regulation; DNA-binding; Transcription factor; C2H2-type zinc finger protein. Chromosomal Location of Human Ortholog: Xp11.23. Cellular Component: protein complex; cytoplasm; nucleus. Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; histone acetyltransferase binding; NF-kappaB binding; protein binding; NFAT protein binding; protein homodimerization activity; sequence-specific DNA binding; histone deacetylase binding; metal ion binding; transcription corepressor activity; transcription factor activity. Biological Process: negative regulation of interleukin-10 production; positive regulation of transcription, DNA-dependent; myeloid cell homeostasis; negative regulation of cytokine secretion; T cell receptor signaling pathway; negative regulation of interleukin-2 production; negative regulation of interleukin-6 production; T cell homeostasis; tolerance induction to self antigen; negative regulation of transcription factor activity; response to virus; negative regulation of immune response; cytokine production; negative regulation of T cell cytokine production; positive regulation of peripheral T cell tolerance induction; inhibition of NF-kappaB transcription factor; regulation of T cell anergy; negative regulation of interleukin-5 production; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; inhibition of CREB transcription factor; negative regulation of cytokine biosynthetic process; transcription from RNA polymerase II promoter; negative regulation of interleukin-4 production; T cell activation; negative regulation of activated T cell proliferation; negative regulation of transcription from RNA polymerase II promoter; negative regulation of interferon-gamma biosynthetic process; negative regulation of histone acetylation; positive regulation of interleukin-4 production; negative regulation of cell proliferation; negative regulation of isotype switching to IgE isotypes; CD4-positive, CD25-positive, alpha-beta regulatory T cell lineage commitment; negative regulation of T cell proliferation; regulation of transcription, DNA-dependent; B cell homeostasis; negative regulation of interferon-gamma production; regulation of isotype switching to IgG isotypes; positive regulation of T cell anergy; positive regulation of transforming growth factor-beta1 production; negative regulation of chronic inflammatory response; negative regulation of histone deacetylation; T cell mediated immunity; positive regulation of immature T cell proliferation in the thymus; negative regulation of tumor necrosis factor production; chromatin remodeling; negative regulation of interleukin-17 production; positive regulation of CD4-positive, CD25-positive, alpha-beta regulatory T cell differentiation; negative regulation of interleukin-2 biosynthetic process; positive regulation of histone acetylation. Disease: Diabetes Mellitus, Insulin-dependent; Immunodysregulation, Polyendocrinopathy, And Enteropathy, X-linked

size1 :

24-Strip-Wells

price1 :

215 USD

size2 :

48-Strip-Wells

price2 :

410

size3 :

96-Strip-Wells

price3 :

490

size4 :

5x96-Strip-Wells

price4 :

2040

size5 :

10x96-Strip-Wells

price5 :

3590