ELISA/assay

Rat TRACP-5b (Tartrate-Resistant Acid Phosphatase 5b) ELISA Kit

MBS2510027

24-Strip-Wells

215 USD

ELISA Kit

Rat TRACP-5b (Tartrate-Resistant Acid Phosphatase 5b) ELISA Kit

[TRACP-5b]

[tartrate-resistant acid phosphatase type 5; Tartrate-resistant acid phosphatase type 5; tartrate-resistant acid phosphatase type 5; TrATPase; tartrate-resistant acid ATPase; acid phosphatase 5, tartrate resistant; Tartrate-resistant acid ATPase; TrATPase; Type 5 acid phosphatase]

[TRACP-5b]

[ACP5; ACP5; TRAP; SPENCDI; TR-AP; TrATPase]

PPA5_HUMAN

Rat

325

This kit recognizes Rat ACE in samples. No significant cross-reactivity or interference between Rat ACE and analogues was observed.

Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 0.31-20ng/mL. Sensitivity: 0.19ng/mL.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat ACE were tested 20 times on one plate, respectively. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat ACE were tested on 3 different plates, 20 replicates in each plate.

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Rat ACE concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat ACE. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat ACE and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat ACE, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Rat ACE. You can calculate the concentration of Rat ACE in the samples by comparing the OD of the samples to the standard curve.

161377455

NP_001104506.1

NM_001111036.1

P13686

36,599 Da

Defective AMN Causes Hereditary Megaloblastic Anemia 1 Pathway (906000); Defective BTD Causes Biotidinase Deficiency Pathway (906015); Defective CD320 Causes Methylmalonic Aciduria Pathway (906012); Defective CUBN Causes Hereditary Megaloblastic Anemia 1 Pathway (906001); Defective GIF Causes Intrinsic Factor Deficiency Pathway (906004); Defective HLCS Causes Multiple Carboxylase Deficiency Pathway (906014); Defective LMBRD1 Causes Methylmalonic Aciduria And Homocystinuria Type CblF Pathway (906003); Defective MMAA Causes Methylmalonic Aciduria Type CblA Pathway (906010); Defective MMAB Causes Methylmalonic Aciduria Type CblB Pathway (906009); Defective MMACHC Causes Methylmalonic Aciduria And Homocystinuria Type CblC Pathway (906005)

This gene encodes an iron containing glycoprotein which catalyzes the conversion of orthophosphoric monoester to alcohol and orthophosphate. It is the most basic of the acid phosphatases and is the only form not inhibited by L(+)-tartrate. [provided by RefSeq, Aug 2008]

ACP5: Involved in osteopontin/bone sialoprotein dephosphorylation. Its expression seems to increase in certain pathological states such as Gaucher and Hodgkin diseases, the hairy cell, the B-cell, and the T-cell leukemias. Defects in ACP5 are the cause of spondyloenchondrodysplasia with immune dysregulation (SPENCDI). A disease characterized by vertebral and metaphyseal dysplasia, spasticity with cerebral calcifications, and strong predisposition to autoimmune diseases. The skeletal dysplasia is characterized by radiolucent and irregular spondylar and metaphyseal lesions that represent islands of chondroid tissue within bone. ACP5 inactivating mutations result in a functional excess of phosphorylated osteopontin causing deregulation of osteopontin signaling and consequential autoimmune disease. Belongs to the metallophosphoesterase superfamily. Purple acid phosphatase family. Protein type: Phosphatase; EC 3.1.3.2; Cofactor and Vitamin Metabolism - riboflavin; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 19p13.2. Cellular Component: lysosome; integral to membrane; cytosol. Molecular Function: acid phosphatase activity; ferric iron binding; ferrous iron binding. Biological Process: negative regulation of interleukin-12 production; vitamin metabolic process; negative regulation of nitric oxide biosynthetic process; response to lipopolysaccharide; negative regulation of interleukin-1 beta production; negative regulation of tumor necrosis factor production; negative regulation of superoxide release; riboflavin metabolic process; defense response to Gram-positive bacterium; dephosphorylation; negative regulation of inflammatory response; response to cytokine stimulus; water-soluble vitamin metabolic process; bone resorption. Disease: Spondyloenchondrodysplasia With Immune Dysregulation

24-Strip-Wells

215 USD

48-Strip-Wells

410

96-Strip-Wells

490

5x96-Strip-Wells

2040

10x96-Strip-Wells

3590