ELISA/assay

Monkey MASP1 (Mannan Associated Serine Protease 1) ELISA Kit

MBS2510001

24-Strip-Wells

235 USD

ELISA Kit

Monkey MASP1 (Mannan Associated Serine Protease 1) ELISA Kit

[MASP1]

[MASP1 protein, partial; Mannan-binding lectin serine protease 1; mannan-binding lectin serine protease 1; Ra-reactive factor serine protease p100; complement factor MASP-3; complement-activating component of Ra-reactive factor; mannose-binding lectin-associated serine protease 1; mannose-binding protein-associated serine protease; serine protease 5; mannan-binding lectin serine peptidase 1 (C4/C2 activating component of Ra-reactive factor); Complement factor MASP-3; Complement-activating component of Ra-reactive factor; Mannose-binding lectin-associated serine protease 1; MASP-1; Mannose-binding protein-associated serine protease; Ra-reactive factor serine protease p100; RaRF; Serine protease 5Cleaved into the following 2 chains:Mannan-binding lectin serine protease 1 heavy chain; Mannan-binding lectin serine protease 1 light chain]

[MASP1]

[MASP1; MASP1; 3MC1; MAP1; MASP; RaRF; CRARF; MASP3; MAp44; PRSS5; CRARF1; CRARF; CRARF1; PRSS5; MASP-1; RaRF]

MASP1_HUMAN

Monkey

488

This kit recognizes natural and recombinantMonkeyMASP1. No significant cross-reactivity or interference between MonkeyMASP1 and analogues was observed.

Store at 4 degree C.

Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 0.156-10ng/mL . Sensitivity: Min: 0.094ng/mL ; Max: 10ng/mL

Intended Uses: This ELISA kit applies to the invitro quantitative determination of MonkeyMASP1concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to MASP1. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for MASP1and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain MASP1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of MASP1.You can calculate the concentration of MASP1in the samples by comparing the OD of the samples to the standard curve.

24981014

AAH39724.1

P48740

68,918 Da

Binding And Uptake Of Ligands By Scavenger Receptors Pathway (771599); Complement Activation, Classical Pathway (198823); Complement And Coagulation Cascades Pathway (198880); Complement And Coagulation Cascades Pathway (83073); Complement And Coagulation Cascades Pathway (484); Complement Cascade Pathway (106405); Creation Of C4 And C2 Activators Pathway (106407); Ficolins Bind To Repetitive Carbohydrate Structures On The Target Cell Surface Pathway (833818); Immune System Pathway (106386); Initial Triggering Of Complement Pathway (106406)

This gene encodes a serine protease that functions as a component of the lectin pathway of complement activation. The complement pathway plays an essential role in the innate and adaptive immune response. The encoded protein is synthesized as a zymogen and is activated when it complexes with the pathogen recognition molecules of lectin pathway, the mannose-binding lectin and the ficolins. This protein is not directly involved in complement activation but may play a role as an amplifier of complement activation by cleaving complement C2 or by activating another complement serine protease, MASP-2. The encoded protein is also able to cleave fibrinogen and factor XIII and may may be involved in coagulation. A splice variant of this gene which lacks the serine protease domain functions as an inhibitor of the complement pathway. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Apr 2010]

MASP1: Functions in the lectin pathway of complement, which performs a key role in innate immunity by recognizing pathogens through patterns of sugar moieties and neutralizing them. The lectin pathway is triggered upon binding of mannan-binding lectin (MBL) and ficolins to sugar moieties which leads to activation of the associated proteases MASP1 and MASP2. Functions as an endopeptidase and may activate MASP2 or C2 or directly activate C3 the key component of complement reaction. Isoform 2 may have an inhibitory effect on the activation of the lectin pathway of complement or may cleave IGFBP5. Defects in MASP1 are the cause of 3MC syndrome type 1 (3MC1). 3MC1 is a disorder characterized by facial dysmorphism that includes hypertelorism, blepharophimosis, blepharoptosis and highly arched eyebrows, cleft lip and/or palate, craniosynostosis, learning disability and genital, limb and vesicorenal anomalies. The term 3MC syndrome includes Carnevale, Mingarelli, Malpuech, and Michels syndromes. Belongs to the peptidase S1 family. 4 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted, signal peptide; Protease; Secreted; EC 3.4.21.-. Chromosomal Location of Human Ortholog: 3q27-q28. Cellular Component: extracellular space; extracellular region. Molecular Function: peptidase activity; protein binding; protein homodimerization activity; serine-type endopeptidase activity; calcium ion binding; calcium-dependent protein binding. Biological Process: receptor-mediated endocytosis; negative regulation of complement activation; innate immune response; complement activation, lectin pathway; proteolysis; complement activation. Disease: 3mc Syndrome 1

24-Strip-Wells

235 USD

48-Strip-Wells

450

96-Strip-Wells

535

5x96-Strip-Wells

2255

10x96-Strip-Wells

3975