catalog number :
MBS2509909
products type :
ELISA Kit
products full name :
Rat AMH (Anti-Mullerian Hormone) ELISA Kit
products short name :
AMH
other names :
Amh protein, partial; Muellerian-inhibiting factor; muellerian-inhibiting factor; Mullerian inhibiting substance; anti-Muellerian hormone; muellerian-inhibiting substance; anti-Mullerian hormone; Anti-Muellerian hormone; AMH; Muellerian-inhibiting substance; MIS
other gene names :
Amh; Amh; MIS; AMH; MIS
uniprot entry name :
MIS_MOUSE
specificity :
This kit recognizes natural and recombinant Rat AMH. No significant cross-reactivity or interference between Rat AMH and analogues was observed.
storage stability :
Store at 4 degree C.
other info1 :
Samples: Serum, plasma and other biological fluids. Assay Type: Sandwich. Detection Range: 0.156-10ng/mL. Sensitivity: The minimum detectable dose of Rat AMH is 0.094ng/mL (The sensitivity of this assay, or lowest detectable limit (LDL) was defined as the lowest protein concentration that could be differentiated from zero).
other info2 :
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Rat AMH concentrations in serum, plasma and other biological fluids.
products description :
Principle of the assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to AMH. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for AMH and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain AMH, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of AMH. You can calculate the concentration of AMH in the samples by comparing the OD of the samples to the standard curve.
ncbi acc num :
AAI50478.1
ncbi mol weight :
59,778 Da
ncbi pathways :
BMP Signaling Pathway (1084868); BMP Signaling Pathway (1108218); Cytokine-cytokine Receptor Interaction Pathway (83248); Cytokine-cytokine Receptor Interaction Pathway (460); Hippo Signaling Pathway (749786); Hippo Signaling Pathway (750388); TGF-beta Signaling Pathway (83261); TGF-beta Signaling Pathway (475); CAMP Signaling Pathway (1017641); CAMP Signaling Pathway (1019520)
ncbi summary :
This gene is a member of the transforming growth factor-beta superfamily of growth and differentiation factors. The encoded protein is produced in fetal Sertoli cells during male gonadal differentiation. The protein binds to the anti-Mullerian hormone receptor type 2 and causes the regression of Mullerian ducts in developing male embryos. In humans, some mutations in this gene are associated with persistent Mullerian duct syndrome (PMDS). [provided by RefSeq, Apr 2013]
uniprot summary :
AMH: This glycoprotein, produced by the Sertoli cells of the testis, causes regression of the Muellerian duct. It is also able to inhibit the growth of tumors derived from tissues of Muellerian duct origin. Defects in AMH are the cause of persistent Muellerian duct syndrome type 1 (PMDS1). PMDS1 is a form of male pseudohermaphroditism characterized by a failure of Muellerian duct regression in otherwise normal males. Belongs to the TGF-beta family. Protein type: Secreted; Secreted, signal peptide. Cellular Component: extracellular space; cytoplasm. Molecular Function: hormone activity; transforming growth factor beta receptor binding; receptor binding. Biological Process: Mullerian duct regression; cell-cell signaling; preantral ovarian follicle growth; urogenital system development; activation of NF-kappaB transcription factor