catalog number :
MBS2509844
products type :
ELISA Kit
products full name :
Human CCL4L1 (Chemokine C-C-Motif Ligand 4 Like Protein 1) ELISA Kit
products short name :
[CCL4L1]
other names :
[C-C motif chemokine 4-like; C-C motif chemokine 4-like; chemokine (C-C motif) ligand 4-like 2; Lymphocyte activation gene 1 protein; LAG-1; Macrophage inflammatory protein 1-beta; MIP-1-beta; Monocyte adherence-induced protein 5-alpha; Small-inducible cytokine A4-like]
products gene name :
[CCL4L1]
other gene names :
[CCL4L2; CCL4L1; CCL4L; SCYA4L; AT744.2; SCYQ4L2; CCL4L; LAG1; SCYA4L1; LAG-1; MIP-1-beta]
uniprot entry name :
CC4L_HUMAN
specificity :
This kit recognizes Chicken BALP in samples. No significant cross-reactivity or interference between ChickenBALPand analogues was observed.
storage stability :
Store at 4 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 0.313-20ng/mL. Sensitivity: 0.188ng/mL.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, midrange and high level ChickenBALPwere tested 20 times on one plate, respectively. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, midrange and high level ChickenBALPwere testedon 3 different plates, 20 replicates in each plate.
products description :
Intended Uses: This ELISA kit applies to the invitro quantitative determination of ChickenBALPconcentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to BALP. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for BALPand Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain BALP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/-2 nm. The OD value is proportional to the concentration of BALP.You can calculate the concentration of BALPin the samples by comparing the OD of the samples to the standard curve.
ncbi acc num :
NP_001001435.1
ncbi gb acc num :
NM_001001435.2
ncbi mol weight :
11,008 Da
ncbi pathways :
Chemokine Signaling Pathway (99051); Chemokine Signaling Pathway (96864); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Cytosolic DNA-sensing Pathway (125137); Cytosolic DNA-sensing Pathway (124833); NF-kappa B Signaling Pathway (634527); Salmonella Infection Pathway (375172); Salmonella Infection Pathway (375149); Toll-like Receptor Signaling Pathway (83076)
ncbi summary :
This gene is one of several cytokine genes that are clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins that function in inflammatory and immunoregulatory processes. The protein encoded by this family member is similar to the chemokine (C-C motif) ligand 4 product, which inhibits HIV entry by binding to the cellular receptor CCR5. The copy number of this gene varies among individuals, where most individuals have one to five copies. This gene copy contains a non-consensus splice acceptor site at the 3' terminal exon found in other highly similar gene copies, and it thus uses other alternative splice sites for the 3' terminal exon, resulting in multiple transcript variants. [provided by RefSeq, Apr 2014]
uniprot summary :
CCL4L1: Chemokine that induces chemotaxis of cells expressing CCR5 or CCR1. Inhibits HIV replication in peripheral blood monocytes that express CCR5. Belongs to the intercrine beta (chemokine CC) family. 10 isoforms of the human protein are produced by alternative splicing. Protein type: Motility/polarity/chemotaxis; Chemokine; Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 17q12
size5 :
10x96-Strip-Wells